| Caco-2细胞对负载维生素D_3的纳米粒的摄入研究 |
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| 引用本文： | 高艳丽,刘赛. Caco-2细胞对负载维生素D_3的纳米粒的摄入研究[J]. 中国药理学通报, 2011, 27(11): 1504-1509. DOI: 10.3969/j.issn.1001-1978.2011.11.008 |
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| 作者姓名： | 高艳丽 刘赛 |
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| 作者单位： | 1. 临沂市沂水中心医院临床药学科,山东,沂水,276400
2. 青岛大学医学院药理学教研室,山东,青岛,266021 |
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| 基金项目： | 国家高技术研究发展计划(863计划)支持项目 |
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| 摘 要： | 目的研究Caco-2细胞对负载维生素D3的、FITC标记的自组装海藻酸钠纳米粒(sSAN-VD3-FITC)的摄取及其影响因素,分析其通过Caco-2细胞吸收模型的跨膜转运特点。方法①采用激光共聚焦显微镜检测sSAN-VD3-FITC的摄取,分析作用时间的影响;②采用Caco-2细胞模型研究其跨膜转运,检测接受液的荧光强度,观察其转运特点。计算表观渗透系数(Papp),分析作用时间对转运的影响。结果①sSAN-VD3-FITC作用1 h后细胞质内出现荧光颗粒,4 h后荧光强度增强。②转运接受液的荧光强度、累积转运量较空白对照组均明显升高(P<0.05),均存在时间依赖性(P<0.05)。Papp较稳定,但随作用时间的延长逐渐减小。结论 sSAN-VD3可被Caco-2细胞摄取,并受作用时间的影响;可通过Caco-2细胞模型进行跨膜转运,并存在时间依赖性,表明其可经胃肠道给药吸收入血。
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| 关 键 词： | Caco-2细胞 自组装海藻酸钠纳米粒 维生素D3 FITC 摄取 跨膜转运 |
The uptake of vitamin D3 imbed by self-assembled sodium alginate nanoparticles with caco-2 cells |
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| GAO Yan-li,LIU Sai. The uptake of vitamin D3 imbed by self-assembled sodium alginate nanoparticles with caco-2 cells[J]. Chinese Pharmacological Bulletin, 2011, 27(11): 1504-1509. DOI: 10.3969/j.issn.1001-1978.2011.11.008 |
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| Authors: | GAO Yan-li LIU Sai |
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| Affiliation: | GAO Yan-li1,LIU Sai2(1.Dept of Clinical Pharmarcy,the Central Hospital of Yishui,Yishui Shandong 276400,China,2.Dept of Pharmacy,the Medical Colledge of Qingdao University,Qingdao Shandong 266021,China) |
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| Abstract: | Aim To investigate the uptake of vitamin D3 imbed by self-assembled sodium alginate nanoparticles labeled by FITC(sSAN-VD3-FITC),and the influential factors with Caco-2 cells.And to study the transmembrane transport of it with Caco-2 cell absorption model.Methods Laser scanning confocal microscope(LSCM) was utilized to exam the uptake of sSAN-VD3-FITC absorbed by Caco-2 cells.The effect of drug incubation time on the uptake was analyzed.Caco-2 cell absorption model was used to study the transmembrane transp... |
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| Keywords: | Caco-2 cell self-assembled sodium alginate nanoparticles vitamin D3 FITC uptake transmembrane transport |
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