| HPLC法测定黄麦合剂中淫羊藿苷的含量 |
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| 引用本文： | 金冠钦,孙黎,苏克剑,沈金芳,杨娟. HPLC法测定黄麦合剂中淫羊藿苷的含量[J]. 中国药师, 2011, 14(11): 1589-1590 |
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| 作者姓名： | 金冠钦 孙黎 苏克剑 沈金芳 杨娟 |
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| 作者单位： | 上海交通大学医学院附属仁济医院药剂科,上海,200001 |
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| 基金项目： | 上海浦东新区科技局基金项目 |
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| 摘 要： | 目的:建立HPLC测定黄麦合剂中淫羊藿苷含量的方法。方法:色谱柱:DiamonsilTM C18（250mm×4.6mm,5μm）,流动相:乙腈-水-醋酸（30:70:1）,检测波长:270nm,柱温:30℃,流速:1.0ml·min-1。结果:淫羊藿苷浓度在3.12～100.00μg·ml-1的范围之内,峰面积与浓度呈良好的线性关系（r=0.9999）。平均回收率为92.65%,RSD为2.57%。结论:本方法简便、快速、准确、重复性好,可用于黄麦合剂中淫羊藿苷的含量测定。
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| 关 键 词： | 淫羊藿苷 高效液相色谱法 黄麦合剂 |
Determination of Icraiin in Huangmai Mixtures by HPLC |
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| Jin Guanqin,Sun Li,Su Kejian,Shen Jinfang,Yang Juan. Determination of Icraiin in Huangmai Mixtures by HPLC[J]. China Pharmacist, 2011, 14(11): 1589-1590 |
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| Authors: | Jin Guanqin Sun Li Su Kejian Shen Jinfang Yang Juan |
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| Affiliation: | ( Department of Pharmacy, Renji Hospital Affiliated to School of Medicine, Shanghai Jiaotong University,Shanghai 200001, China) |
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| Abstract: | Objective： To establish an HPLC method for the determination of icraiin in Huangmai mixtures. Method： DiamonsilTM C ls （250 mm × 4. 6 mm ,5 μm） column was used. The mobile phase consisted of acetonitrile-water-acetic acid （30：70：1 ）. Detection wavelength was 270 nm. The column temperature was 30 ℃. The flow rate was 1.0 ml ·min^-1. Result： lcraiin showed a good linear re- lationship within the range of 3.12-100. 00 μg.ml-1 （r =0. 999 9）. The average recovery was 92. 65% with RSD of 2. 57%. Conclu- sion： The method is feasible with good specificity and reproducibility, and can be used for the determination of ieraiin in Huangmai mixtures. |
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| Keywords: | Icraiin HPLC Huangmai mixture |
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