HPLC法同时测定复方痤疮胶囊中齐墩果酸和熊果酸的含量

目的：建立HPLC法同时测定复方痤疮胶囊中齐墩果酸、熊果酸的含量。方法：采用Waters symmetry-C18色谱柱（250mm×4.6mm,5μm）,流动相为甲醇-水-冰醋酸-三乙胺（87：13：0.04：0.02）,检测波长210nm,柱温为30℃。结果：齐墩果酸在10～80mg·L^-1范围内有良好线性关系（r=0.9996）,平均加样回收率为100.76%（RSD=1.86%）。熊果酸在10～80 mg·L^-1范围内有良好线性关系（r=0.9998）,平均加样回收率为100.63%（RSD=1.48%）。结论：本方法测定复方痤疮胶囊中齐墩果酸、熊果酸的含量,方法简便、准确,结果稳定,可为复方痤疮胶囊的质量评价提供依据。

Determination of Oleanolic Acid and Ursolic Acid in Compound Cuochuang Capsules by HPLC

Objective：To establish a high performance liquid chromatography method for the determination of oleanolic acid and ursolic acid in compound Cuochuang capsules.Method：The separation was performed on a waters symmetry-C18column（250 mm×4.6 mm,5μm）with methanol-water-acetic acid-triethylamine（87：13：0.04：0.02）as the mobile phase at a flow rate of 1.0 ml· min^-1,and UV detection wavelength was at 210 nm.Result：The calibration curve for oleanolic acid was linear within the range of 10- 80 mg·L^-1（r=0.999 8）with the average recovery of（100.76±1.86）%.The calibration curve for ursolic acid was linear within the range of 10-80 mg·L^-1（r=0.999 9）with the average recovery of（100.63±1.48）%.Conclusion：The method is sensitive,accurate and reliable for the quality control of compound Cuochuang capsules.