喉癌手术切缘中Survivin和Caspase-3及p53的表达意义及相关性研究

目的:研究Survivin、Caspase-3及p53在喉鳞状细胞癌手术切缘中的表达意义及相关性.方法:116例患者术后按肿瘤复发情况分成4组,分别为:复发≤1年组,＞1～3年组,＞3～＜5年组,≥5年组(包括5年后无复发),应用免疫组织化学方法检测手术切缘Survivin、Caspase-3及p53的表达情况.结果:Survivin在4组中的表达分别为92.9%、85.0%、50.0%及21.1%(P＜0.01)Caspase-3在4组中的表达分别为50.0%、50.0%、66.7%及89.5%(P＜0.01);p53在4组中的表达分别为57.1%、37.5%、45.8%及18.4%(P＜0.05);Survivin、Caspase-3及p53在4组的表达均有统计学意义.结论:喉癌手术切缘中Survivin、Caspase-3及p53的表达在喉鳞状细胞癌的发生、发展中可能起重要作用,并对喉鳞状细胞癌的早期诊断和预后判断有意义.

Expression of Survivin,Caspase-3 and p53 in surgical margin of laryngeal squamous cell carcinoma and their relationship

Objective:To study the expression of Survivin, Caspase-3 and p53 in surgical margin of laryngeal squamous cell carcinoma(LSCC),and their clinical significiance and relationship.Method:Immunohistochemistry method was used to detect the expression of Survivin,Caspase-3 and p53 in 116 cases of surgical margin of LSCC.Result:According to the recurrence,the patients were divided into four group,recurrence within one year,between one year to three years,between three to five years,more than 5 years(include no recurrence).The expression rate of survivin were 92.9%,85.0%,50.0% and 21.1%(P<0.01) in four groups,respectively.The expression rate of Caspase-3 were 50.0%,50.0%,66.7% and 89.5%(P<0.01).The expression rate of p53 were 57.1%,37.5%,45.8% and 18.4%(P<0.05).The expression rate of survivin,caspase-3 and p53 in four groups,respectively,there were significant differences(P<0.05).Conclusion:Expression of Survivin,Caspase-3 and p53 in surgical margin of LSCC may play an important role in the tumorigenesis and develeopment of laryngeal carcinoma.They are feasible for early diagnosis and prognosis estimation.