| 流产模型孕鼠脾脏调节性T细胞分离纯化方法的初步建立 |
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| 引用本文： | 殷治华,赵富玺,穆雅琴,刘润花,王健,冯婷婷,齐彦.流产模型孕鼠脾脏调节性T细胞分离纯化方法的初步建立[J].中国优生与遗传杂志,2012(3):36-37,30. |
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| 作者姓名： | 殷治华 赵富玺 穆雅琴 刘润花 王健 冯婷婷 齐彦 |
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| 作者单位： | 山西医科大学基础医学院;山西大同大学免疫研究所 |
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| 基金项目： | 国家自然科学基金(81170621);国家人口和计划生育委员会立项项目(C-73);山西省科学技术发展计划项目(20080311072) |
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| 摘 要： | 目的探索流产模型孕鼠CD4＋CD25＋T细胞和CD4＋CD25-T细胞快速高效的分选方法。方法采用免疫磁珠两步法分离流产模型孕鼠脾脏中T细胞,分离后的细胞经台盼蓝染色检测细胞存活率,经流式细胞仪检测分离的纯度。结果流产模型孕鼠脾脏细胞经过免疫磁珠细胞分选法2次分选得到CD4＋/CD25＋调节性T细胞纯度为（93.5±1.1）%,CD4＋/CD25-T细胞纯度为（96.6±0.6）%。结论采用免疫磁珠细胞分选法能够快速高效的得到高纯度的CD4＋CD25＋T细胞和CD4＋CD25-T细胞。
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| 关 键 词： | 流产模型 调节性T细胞 免疫磁珠细胞分选 流式细胞仪检测 |
The preliminary establishment of the separation and purification methods of regulatory T cells in the spleen of abortion model rat |
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| YIN Zhi-hua,ZHAO Fu-xi,MU Ya-qing,LIU Run-hua,WANG Jian,FEN Ting-ting,QI Yan.The preliminary establishment of the separation and purification methods of regulatory T cells in the spleen of abortion model rat[J].Chinese Journal of Birth Health & Heredity,2012(3):36-37,30. |
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| Authors: | YIN Zhi-hua ZHAO Fu-xi MU Ya-qing LIU Run-hua WANG Jian FEN Ting-ting QI Yan |
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| Institution: | .(Preclinical Medical College of Shanxi Medical University,Shanxi Taiyan 030001) |
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| Abstract: | Ojective: To explore the method of separation of CD4+CD25+ T cells and CD4+ CD25-T cells in abortion model pregnant rat fast and effectively.Methods: The immune magnetic beads two-step separation is used to separate T cells of the spleen in abortion model rat,and the cell survival rate is tested by a cell to blue stain after separated,the flow cytometric tests the purity of separation.Results: The purity of CD4+CD25+regulatory T cells is(93.5±1.1) % in abortion model pregnant rat by magnetic beads immune cell separation method for tow times,the purity of CD4+CD25-T cells is(96.6±0.6) %.Conclusion: The immune cells magnetic beads separation method of high speed and high efficiency can get high purity of CD4+CD25+ T cells and CD4+CD25-T cells. |
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| Keywords: | Abortion model Regulatory T cells Immune cell sorting magnetic beads Flow cytometric detection |
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