消栓颗粒对缺氧缺血性新生大鼠脑组织Caspase-3蛋白的影响

目的探讨消栓颗粒对缺氧缺血性脑损伤（HIBD）新生大鼠Caspase-3蛋白的影响。方法 7日龄SD新生大鼠36只,随机分为假手术组、HIBD模型组和消栓颗粒治疗组,每组各12只。HIBD模型组和消栓治疗组参照Yager法建立HIBD模型,消栓颗粒治疗组灌服消栓颗粒（8g/kg,qd）,HIBD模型组及假手术组灌服等量9g/L氯化钠注射液。造模后第14d断头取出脑组织,观察3组大鼠左侧大脑半球病理形态学的变化,比较3组的病理学评分;免疫组化法检测3组大鼠海马及皮质区Caspase-3蛋白的表达情况。结果 HE染色结果显示,假手术组未见明显病变,HIBD模型组大鼠脑组织变性、坏死显著,消栓颗粒治疗组仅表现为局灶性神经细胞变性,病理学评分假手术组显著低于HIBD模型组及消栓颗粒治疗组（P〈0.01）,消栓颗粒组低于HIBD模型组（P〈0.01）。免疫组化结果显示,假手术组海马及皮质区有少量Caspase-3蛋白表达,HIBD模型组Caspase-3蛋白的表达较假手术组明显增多,消栓颗粒治疗组Caspase-3蛋白的表达较HIBD模型组明显减少,3组间两两比较均具有显著性差异（P〈0.01）。结论消栓颗粒可减轻HIBD新生大鼠的脑损伤,机制可能为抑制Caspase-3蛋白的表达。

Effects of Xiao Shuan Ke Li onexpression of Caspase-3 protein in brain tissue of neonatal rats with hypoxic-ischemic brain damage

Objective:To explore the effects of Xiao Shuan Ke Li(XSKL) on Caspase-3 protein neonatal rats with hypoxic-ischemic brain damage(HIBD).Methods:36 seven-day-old Sprague Dawley(SD) neonatal rats were randomly divided into 3 groups:sham operation group(n=12),HIBD model group(n=12),XSKL treatment group(n=12).Animal model of HIBD was induced according to the Yager method.The rats of treatment group were given oral XSKL(8g/kg,qd) for 14 days.HIBD model group and sham operation group were given the same amounts of saline for 14 days.The morphologic changes in the brain were observed,and the quantitative pathological score of three groups were compared.The amounts of Caspase-3 protein in hippocampal and cortex area were observed by immunohistochemical method.Results The brain organization in rats degenerated and necrosised significantly in HIBD model group,while only focal nerve cell degenerated in XSKL treatment group,and rarely in sham operation group.The quantitative pathological score of sham operation group HIBD model group was much lower than those in HIBD model group and XSKL treatment group(P<0.01),while in XSKL treatment group was lower than that in HIBD model group(P<0.01).Immunohistochemical method showed that the amounts of Caspase-3 protein in hippocampal and cortex area were little in sham operation group,and XSKL treatment group were much fewer than those of HIBD model group(P<0.01).Conclusion:XSKL can reduce the brain damage of HIBD neotatal rats,the mechanism may be that XSKL can inhibit the expressin of Caspase-3 protein.