苦参对蓝氏贾第鞭毛虫作用及其机理初步研究

目的研究苦参对蓝氏贾第鞭毛虫的作用及其机理.方法用苦参作用蓝氏贾第鞭毛虫滋养体,观察滋养体脱壁及死亡情况,并用光镜和透射电镜观察其形态和内部结构的变化.结果在作用 6、8和24 h后,滋养体脱壁率分别为47.4%、70.9%和80.2%,死亡率为24.5%、36.1%和54.2%;形态观察可见,苦参作用的滋养体收缩,胞质内容物减少,吸盘部分脱离胞体,有的滋养体部分胞膜内陷.结论苦参在体外能引起滋养体脱壁、死亡,其抗虫作用是肯定的,吸盘脱离、质膜凹陷及胞质减少等,这可能是其对贾第虫作用机理所在.

PRELIMINARY STUDIES ON THE EFFECTS OF A CHINESE HERB-RADIX SOPHORAE FLAVESCENTIS ON TROPHOZOITES OF GIARDIA LAMBLIA IN VITRO

Objective To investigate the effect and mechanism of radix Sophorae flavescentis on the trophozoites of Giardia lamblia in vitro . Methods Trophozoites of G. lamblia were exposed to aqueous extracts of radix S. flavescentis and then the detachment from the tube wall, the death rate and the morphological changes under light and transmission electron microscope of trophozoites were studied. Results After exposure to 1.6% aqueous extracts of radix S. flavescentis for 6, 8 and 24 h, the detachment rates were 47.4%, 70.9% and 80.2%, and the death rates were 24.5%, 36.1 % and 54.2%, respectively. The radix S. flavescentis treated trophozoites shrinked and became roundish. There were also changes in ultrastructure, such as depletion of the contents of cytoplasm, separation of adhesive disk from the cell body and appearance of pit in cell membrane of some treated trophozoites. Conclusion Radix S. flavescentis can kill trophozoites of G. lamblia and lead them to detach from the tube wall in vitro . The induced morphological changes may be partially responsible for the effects of radix S. flavescentis on giardiasis.