| siRNA-FAM92A1-289对HeLa细胞增殖的影响 |
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| 引用本文： | 陈洁,李瑞明,方娟,卢志勇,阮绪芝.siRNA-FAM92A1-289对HeLa细胞增殖的影响[J].郧阳医学院学报,2011,30(2):105-108. |
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| 作者姓名： | 陈洁 李瑞明 方娟 卢志勇 阮绪芝 |
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| 作者单位： | 陈洁,CHEN Jie(湖北医药学院胚胎干细胞研究湖北省重点实验室,湖北,十堰,442000;江西中医学院附属医院病理科,江西,南昌,330006);李瑞明,方娟,卢志勇,阮绪芝,LI Rui-ming,FANG Juan,LU Zhi-yong,RUAN Xu-zhi(湖北医药学院胚胎干细胞研究湖北省重点实验室,湖北,十堰,442000) |
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| 摘 要： | 目的:研究RNA干扰(RNAi)沉默基因FAM92A1-289对HeLa细胞增殖和凋亡的影响。方法:化学合成法合成小干扰RNA(siRNA),阳离子脂质体转染FAM92A1-289 HeLa细胞;实时定量RT-PCR检测其mRNA表达,MTT法和流式细胞仪检测转染后HeLa细胞增殖、细胞周期和凋亡变化。结果:转染合成FAM92A1-289 siRNA后,HeLa细胞中FAM92A1-289 mRNA表达下降(P<0.01),细胞增殖均明显增强(P<0.01);细胞周期分布改变,S期细胞明显增多,而G2/M期细胞减少,凋亡率无变化。结论:FAM92A1-289基因调节细胞增殖活动。
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| 关 键 词： | 小RNA干扰 FAM92A1-289基因 HeLa细胞 |
Influence of siRNA-FAM92A1-289 Gene on Proliferation and Apoptosis of HeLa cells |
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| CHEN Jie,LI Rui-ming,FANG Juan,LU Zhi-yong,RUAN Xu-zhi.Influence of siRNA-FAM92A1-289 Gene on Proliferation and Apoptosis of HeLa cells[J].Journal of Yunyang Medical College,2011,30(2):105-108. |
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| Authors: | CHEN Jie LI Rui-ming FANG Juan LU Zhi-yong RUAN Xu-zhi |
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| Abstract: | Objective To study the influence of RNA interference to repress FAM92A1-289 gene on the proliferation and apoptosis of HeLa cell.Methods siRNA were chemically synthesized and transfected into FAM92A1-289 HeLa cells with lipofectamine.The mRNA expression were detected with real-time quantitive RT-PCR.The cell cycle,apoptosis and proliferation of HeLa cell were detected with flow cytometry and MTT.Results After successfully synthesized and transfected FAM92A1-289 siRNA,the expression level of FAM92A1-289 mRNA were decreased(P<0.01),the cell proliferation level was increased(P<0.01),obviously.The distribution of cell cycle was changed,the number of cells in S phase was increased,but in G2/M phase was decreased,and the apoptosis rate had no change.Conclusion The FAM92A1-289 gene may play an important role in the proliferation of HeLa cell. |
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| Keywords: | siRNA FAM92A1-289 Gene HeLa cell |
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