| GC-MS法测定火麻中△~9-四氢大麻酚的含量 |
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| 引用本文： | 郭宇姝,冯建林,刘勤,张沂,谢剑炜.GC-MS法测定火麻中△~9-四氢大麻酚的含量[J].中国药房,2008,19(3):201-202. |
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| 作者姓名： | 郭宇姝 冯建林 刘勤 张沂 谢剑炜 |
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| 作者单位： | 1. 海军总医院药剂科,北京市,100037
2. 军事医学科学院毒物药物研究所,北京市,100850 |
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| 摘 要： | 目的:建立以气相色谱-质谱法测定火麻壳和火麻仁中Δ9-四氢大麻酚含量的方法。方法:采用HP-5石英毛细管柱,程序升温:200℃(保持1min)~270℃(保持10min),升温速率为3℃·min-1,进样口温度为280℃,检测器温度为290℃。结果:Δ9-四氢大麻酚检测浓度在5.0~45.0μg·mL-1范围内与峰面积比值呈良好的线性关系(r=0.9987);最低检测限为1.0μg·mL-1(S/N=5);平均加样回收率为81.1%,RSD=3.8%(n=9)。结论:本方法简便、准确、重现性好,可用于火麻制品的质量控制。
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| 关 键 词： | 火麻 Δ9-四氢大麻酚 气相色谱-质谱法 含量测定 |
| 文章编号： | 1001-0408（2008）03-0201-02 |
| 收稿时间： | 2007-02-12 |
| 修稿时间： | 2007-04-11 |
Determination of △9- tetrahydrocannabinol Content in Hemp by GC - MS |
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| GUO Yu-shu,FENG Jian-lin,LIU Qin,ZHANG Yi,XIE Jian-wei.Determination of △9- tetrahydrocannabinol Content in Hemp by GC - MS[J].China Pharmacy,2008,19(3):201-202. |
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| Authors: | GUO Yu-shu FENG Jian-lin LIU Qin ZHANG Yi XIE Jian-wei |
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| Institution: | GUO Yu-shu,ZHANG Yi,FENG Jian-lin,LIU Qin,XIE Jian-wei(Dept. of Pharmacy, Naval General Hospital, Beijing 100037, China;Beijing Institute of Pharmacology and Toxicology, Academy of Mil itary Medical Sciences, Beijing 100850,China) |
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| Abstract: | OBJECTIVE： To determine the content of △^9- tetrahydrocannabinol （THC） in hemp by GC-MS. METH ODS： HP- 5 quartz capillary column was used. The temperature programming was as follows： the initial temperature was kept at 200 ℃ for 1 min, then raised to 270 ℃ at the rate of 3 ℃. min ^-1 and kept for 10 min; the injector temperature was 280 ℃ and the detector temperature was 290 ℃. RESULTS： The good linearity between peak area and concentration was obtained over the range of 5.0 -45.0 μg . mL^-1 （ r = 0.998 7）. The lowest detectable limit was 1.0μg . mL^-1（S/N = 5） .The average recovery rate was 81.1% （RSD = 3.8%, n = 9） . CONCLUSION： The procedure is simple, accurate and reproducible, and suitable for the quality control of hemp preparation. |
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| Keywords: | Hemp △^9- tetrahydrocannabinol GC- MS Content determination |
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