| 多药耐药相关蛋白1 mRNA在慢性癫痫大鼠脑中的表达 |
| |
| 引用本文： | 蒋颖,晏玉奎,狄晴.多药耐药相关蛋白1 mRNA在慢性癫痫大鼠脑中的表达[J].医学研究生学报,2010,23(2):145-149. |
| |
| 作者姓名： | 蒋颖 晏玉奎 狄晴 |
| |
| 作者单位： | 南京医科大学附属脑科医院神经内科,南京,210029 |
| |
| 基金项目： | 南京市医学科技发展重大项目(2007-0211) |
| |
| 摘 要： | 目的难治性癫痫（refractory epilepsy，RE）的发生机制尚不明确。现已明确多药耐药相关蛋白（multidrug resistance associated protein，MRP）1与某些肿瘤耐药有关。为了探讨该蛋白是否与癫痫的多药耐药相关，本研究利用大鼠癫痫模型观察癫痫发作对大鼠脑MRP1 mRNA表达的影响及左乙拉西坦（Levetiracetam，LEV）和托吡酯（Topiramate，TPM）的作用。方法通过脑立体定向技术，将海人酸（kainic acid，KA）1．5μg（3μl）注射至SD大鼠海马，制作癫痫模型。对照组大鼠海马注射3μl等渗盐水。建立模型3d后，将癫痫组和对照组大鼠各15只随机分为LEV、TPM和Ns亚组分别给予LEV（50mg／kg）、TPM（40mg／kg）和等体积NS灌胃，1次／d，共30d。用荧光定量RT—PCR测定大鼠颞叶、海马MRP1 mRNA表达水平，并进行定量分析。结果各组癫痫大鼠颞叶、海马MRP1 mRNA表达与相应非癫痫组相比呈增高趋势，但差异无统计学意义。非癫痫或癫痫大鼠各处理组间颞叶或海马MRP1 mRNA表达水平总体差异均无统计学意义。结论病程为1个月的慢性癫痫大鼠脑内MRP1 mRNA表达无明显增加，TPM、LEV不影响正常和癫痫大鼠脑MRP1 mRNA的表达。
|
| 关 键 词： | 癫痫 海人酸 多药耐药相关蛋白1 mRNA 托吡酯 左乙拉西坦 |
Expression of multidrug resistance associated protein 1 mRNA in the brain of chronic epileptic rats |
| |
| JIANG Ying,YAN Yu-kui,DI Qing.Expression of multidrug resistance associated protein 1 mRNA in the brain of chronic epileptic rats[J].Bulletin of Medical Postgraduate,2010,23(2):145-149. |
| |
| Authors: | JIANG Ying YAN Yu-kui DI Qing |
| |
| Institution: | Department of Neurology;Brain Hospital Affiliated to Nanjing Medical University;Nanjing 210029;Jiangsu;China |
| |
| Abstract: | Objective The pathogenesis of refractory epilepsy(RE) is not yet understood.Multidrug resistance associated protein 1(MRP1) has been shown to correlate with the resistance to some anticancer drugs.Does MRP1 also play a role in the resistance to antiepileptic drugs? The aim of this study is to investigate the influence of spontaneous seizures,Levetiracetam(LEV) and Topiramate(TPM) on the MRP1 mRNA expression in the brain of chronic epileptic rats.Methods Epileptic rat models(n = 15) were made by stereotaxic ... |
| |
| Keywords: | Epilepsy Kainic acid Multidrug resistance associated protein 1 mRNA Levetiracetam Topiramate |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
