功能矫形前伸下颌后大鼠髁突软骨细胞睾酮的分布变化规律

目的　研究快速生长期大鼠髁突软骨细胞睾酮的表达,并探讨功能矫形对髁突软骨细胞睾酮表达的影响。方法　实验组戴自制模拟的功能矫治器引导大鼠下颌前伸,在实验第3天、1、2、3及4周处死大鼠,应用免疫组织化学SP法检测睾酮在髁突软骨细胞上的表达特点。结果　①实验组、对照组大鼠髁突软骨细胞各层都有睾酮的阳性免疫染色,大鼠各层细胞中以成熟层及移行层阳性强度较高,明显高于生发层及过渡层,其中成熟层染色强度最高;②随着大鼠的青春期生长发育,髁突软骨细胞各层睾酮染色强度出现不同的变化特点;③实验第2及第 3周组大鼠髁突成熟层软骨细胞睾酮染色强度明显高于对照各组,差异具有统计学意义(P<0·05)。结论　睾酮参与了大鼠髁突软骨细胞成熟的调控,并介导了功能矫形的机械刺激作用,使髁突软骨细胞的增生分化功能增强,髁突软骨增生。

Expression of Testosterone in the Rapid Growing Rat Condylar Cartilage and the Expression Changes after the Function Mandibular Advacement

OBJECTIVE: To investigate the expressions of Testosterone in the condylar cartilage of rapid growing rats. METHODS: The mimic functional appliances were used in experiment groups and rats were killed after 3 days, 1 week, 2 weeks, 3 weeks and 4 weeks. Immunohistochemical technique was used to detect the expression of T. RESULTS: T was expressed throughout the condylar cartilage. The Highest expressions were in mature layers of both experiment and control groups. Along with the maturation, the expressions of T in the condylar cartilage were changed respectively, and the immunostain levels in the mature layer of experiment groups 2 and 3 weeks were higher than those of control groups (P < 0.05). CONCLUSION: 1. T is a member of cartilage growth and development control system. 2. The orthopedic mechanism of functional appliance may be conducted by T.