Analysis of varicella-zoster virus and herpes simplex virus in various clinical samples by the use of different PCR assays |
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Authors: | Niklas Finnstr m, Karin Bergsten, Helena Str m, Therese Sundell, Sophie Martin, Peter Wutzler,Andreas Sauerbrei |
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Affiliation: | aCepheid Europe, Maurens Scopont, France;bCepheid AB, Bromma, Sweden;cInstitute of Virology and Antiviral Therapy, Friedrich-Schiller University of Jena, Hans-Knoell-Strasse 2, 07745 Jena, Germany |
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Abstract: | Rapid and reliable detection of varicella-zoster virus (VZV) and herpes simplex virus type 1 (HSV-1) and -2 (HSV-2) is of clinical significance in immunocompromised patients and patients with infections of the central nervous system. This paper describes the detection of VZV and HSV using the commercially available Affigene® VZV and Affigene® HSV 1/2 tracer kits in comparison to “in-house” polymerase chain reaction (PCR) assays. For sample preparation, Qiagen (Hilden, Germany) and Affigene® (Cepheid AB, Bromma, Sweden) DNA extraction kits were used. 175 samples were analyzed for VZV and 352 samples for HSV-1 and -2. Generally more positive results were obtained using the Affigene® assays compared to the “in-house” methods independent of the DNA preparation method used. There were significant differences in sensitivity between the Affigene® HSV 1/2 tracer and the “in-house” PCR assays for the detection of both HSV-1 and -2 in cerebrospinal fluid and vesicle/skin swabs. The Affigene® HSV 1/2 and VZV tracers are very sensitive assays for detection of VZV and HSV. A wide variety of clinical samples can be examined in combination with either the Qiagen or the Affigene® DNA extraction kits for preparation. |
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Keywords: | Herpes simplex virus type 1 Herpes simplex virus type 2 Varicella-zoster virus Polymerase chain reaction Virological diagnosis |
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