总谷胱甘肽的测定方法

本文介绍了测定谷胱甘肽总含量的循环法。原理是,在NADPH和GR维持谷胱甘肽总量不变的条件下,GSH和D7NB反应,生成TNB的速率与样品中总谷胱甘肽量呈正比。由于采用了γ-GT抑制剂和快速测定,克服了人血浆中谷胱甘肽含量极低,离体后消退极快,不易准确测定的困难。本法灵敏度可达0.1μM左右,回收率为93～106％。

METHOD FOR DETERMINATION OF TOTAL GLUTATHIONE

The method of recirculating assay for total glutathione is presented in this paper. The principle is that the concentration of total glutathione is proportional to TNB-forming rate resulting from the reaction between GSH and DTNB provided that NADPH and GR are used to keep the amount of total glutathione constant. It is very difficult to get accurate concentrations of glutathione in human plasma because of the very low concentration and the extremely fast disappearance after the blood was drawn. These difficulties are overcome by means of fast measurement and using y~GT inhibitor. The sensitivity of this method is about 0.1 uM and the recovery is 93-106%.