| 百合鳞叶总DNA提取方法的改进 |
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| 引用本文： | 刘湘丹,周日宝,童巧珍,潘清平,高彦宁.百合鳞叶总DNA提取方法的改进[J].湖南中医药大学学报,2007,27(2):12-14. |
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| 作者姓名： | 刘湘丹 周日宝 童巧珍 潘清平 高彦宁 |
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| 作者单位： | 1. 湖南中医药大学2004级硕士研究生班,湖南,长沙,410007
2. 湖南中医药大学药学院,湖南,长沙,410007 |
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| 基金项目： | 国家“十五”科技攻关课题(2004BA721A27) |
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| 摘 要： | 目的建立一种适合RAPD分析的百合鳞叶总DNA提取方法。方法在传统CTAB提取方法上,对其进行改良,进一步优化提取方法。结果用改良CTAB法提取的总DNA的OD260/OD280在1.6-2.0之间,电泳条带清晰,其含量和纯度完全满足RAPD分析的要求。结论该方法提取百合鳞叶总DNA简便实用。
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| 关 键 词： | 百合鳞叶 改良CTAB DNA提取 |
| 文章编号： | 1000-5633（2007）02-0012-03 |
| 修稿时间： | 2006-09-15 |
Improvement of Extracting Total DNA from Lily's Quama Leaves |
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| LIU Xiang-dan,ZHOU Ri-bao,TONG Qiao-zhen,PAN Qing-ping,GAO Yan-ning.Improvement of Extracting Total DNA from Lily''''s Quama Leaves[J].Journal of Traditional Chinese Medicine University of Hunan,2007,27(2):12-14. |
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| Authors: | LIU Xiang-dan ZHOU Ri-bao TONG Qiao-zhen PAN Qing-ping GAO Yan-ning |
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| Institution: | TCM University of Hunan, Changsha, Hunan 410007, China |
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| Abstract: | Objective To excogitate a method for extracting DNA from Bulbus Lilii to be appropriate for RAPD analysis.Methods Based the traditional CTAB,the method was improved to obtain an extrcting method.Results The OD260/OD280 of total DNA was between 1.6 to 2.0 with the improved method of CTAB,the banding patterns was clear,and the content and pureness of the total DNA can meet the reaction for RAPD.Conclusions A simple and useful method for DNA extraction from lily'squama leaves are created. |
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| Keywords: | lily quama leave DNA extraction improved CTAB |
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