大鼠电击伤后血清CK和LDH变化与损伤时间关系的研究

目的：研究大鼠在电击伤后血清中乳酸脱氢酶（LDH）和肌酸激酶（CK）含量的变化与损伤时间的关系,为法医学上电击伤损伤时间的推断提供依据。方法：建立大鼠电击损伤模型,电击伤组于电击后0、1、2、4、8、12h和1、2、3d麻醉抽取5ml心室血,应用全自动生化分析仪检测样本中CK和LDH酶活性。并对数据进行统计学处理。结果：电击伤后大鼠血清中LDH和CK酶活性出现动态变化,呈现一定的规律,反映出电击损伤发生的时间,LDH活性在电击损伤早期（电击伤后4、8、12h）明显升高,CK活性在电击伤后8h达到高峰,之后逐渐下降。结论：电击伤后血清中CK和LDH含量变化,可以为电击损伤时间的推断提供理论依据。

Experimental studies on the content of CK and LDH in rata serum raring With time after electrical injury

Objective：To explore the contents of CK and LDH in rats serum varying with time after electrical injury,and provide medicolegal basis of infer the time of electrical injury.Methods：The electrical injury model established,electrical injury group rats,ventricle blood was extracted after the anesthesia during 0,1,2,4,8,12 h and 1,2,3 d.Then autobiochemistry instrument was used to test CK and LDH contents.Data were brought through statistic test.Results：After electrical injury the contents of CK and LDH in rats serum changed with time with a regular pattern.In early time （4,8,12 h after electrical injury）,the contents LDH increased significantly.While the peak of CK was in 8 h.Conclusion：After electrical injury the change of contents of CK and LDH in rats serum may provide medicolegal basis of identifying electric shock time.