果糖二磷酸钠镁对缺血突触体乳酸含量及ATP酶活性的影响

目的 :研究果糖二磷酸钠镁 (FDPM)对缺血突触体乳酸含量及ATP酶活性的影响 ,以探讨其脑保护作用机制。方法 :制备正常大鼠脑突触体 ,氧糖剥夺培养建立缺血突触体模型 ,分别加 1.3mmol·L- 1硫酸镁 ,4 .0mmol·L- 11,6 二磷酸果糖 (FDP)及 1.3mmol·L- 1果糖二磷酸钠镁共培养 6 0min ,测定突触体乳酸含量及Na+ K+ATP酶和Ca2 + Mg2 +ATP酶活性。结果 :FDPM可明显降低缺血突触体乳酸含量 ,升高Na+ K+ATP酶和Ca2 + Mg2 +ATP酶活性(P <0 .0 5 ) ,其作用强于FDP的作用。结论 :FDPM保护脑缺血的作用机制可能与其改善脑缺血后能量代谢 ,减轻脑组织酸中毒状态有关

Effects of sodium magnesium fructose diphosphate on lactate concentration and ATPase activity of ischemic synaptosome

To study the effects of sodium magnesium fructose diphosphate (FDPM) on lactate concentration and ATPase activity of ischemic synaptosome, so as to explore the protective mechanisms of FDPM on cerebral ischemia. METHODS: Synaptosome prepared from normal rat brain was cultured with oxygen and glucose deprived to establish the model of ischemic synaptosome. 1.3 mmol*L-1 magnesium sulfate, 4.0 mmol*L-1 fructose-1,6-diphosphate (FDP) and 1.3 mmol*L-1 FDPM were incubated with the ischemic synaptosomes separately. After 60 min, the lactate concentration as well as the activities of Na+-K+ ATPase and Ca2+-Mg2+ ATPase were detected. RESULTS: FDPM reduced the lactate concentration, raised the activities of Na+-K+ ATPase and Ca2+-Mg2+ ATPase of ischemic synaptosomes (P<0.05). The effects of FDPM were stronger than those of FDP (P<0.05). CONCLUSION: FDPM may protect cerebral ischemia by improving the energy metabolism, and mitigating acidosis state of brain tissue.