| PTEN基因联合多西环素对黏液表皮样癌细胞侵袭的抑制作用 |
| |
| 引用本文： | 刘斌,徐小方,关素敏,徐海燕,安然.PTEN基因联合多西环素对黏液表皮样癌细胞侵袭的抑制作用[J].口腔医学研究,2010,26(5):617-619. |
| |
| 作者姓名： | 刘斌 徐小方 关素敏 徐海燕 安然 |
| |
| 作者单位： | 第四军医大学口腔医学院生物学教研室;第四军医大学口腔医学院实验动物中心; |
| |
| 摘 要： | 目的：探讨PTEN抑癌基因联合多西环素对高转移性涎腺黏液表皮样癌细胞系体外粘附、迁移和侵袭的影响。方法：用脂质体将野生型PTEN基因导入人黏液表皮样癌细胞系,再用10mg/L多西环素处理细胞3d,采用MTT比色法测定细胞粘附,划伤愈合实验测定细胞迁移,趋化Transwell法测定细胞侵袭。结果：PTEN基因转染和Dox分别处理黏液表皮样癌细胞导致癌细胞粘附、迁移和侵袭受到不同程度的抑制。PTEN基因转染联合多西环素对癌细胞的抑制作用更强,癌细胞在Metrigel和Fn基质上的粘附抑制率分别为37.4%和70.6%;癌细胞迁移抑制率为60.9%;癌细胞侵袭抑制率为65.1%。结论：PTEN抑癌基因联合多西环素对黏液表皮样癌细胞系体外粘附、迁移和侵袭具有显著的协同抑制效应。
|
| 关 键 词： | PTEN基因 多西环素 黏液表皮样癌细胞 粘附 迁移 侵袭 |
Inhibitory Effects of Combination PTEN Gene with Doxycycline on in Vitro Invasion of Human Mucoepidermoid Carcinoma Cell Line |
| |
| LIU Bin,XU Xiao-fang,GUAN Su-min,et al..Inhibitory Effects of Combination PTEN Gene with Doxycycline on in Vitro Invasion of Human Mucoepidermoid Carcinoma Cell Line[J].Journal of Oral Science Research,2010,26(5):617-619. |
| |
| Authors: | LIU Bin XU Xiao-fang GUAN Su-min |
| |
| Institution: | LIU Bin,XU Xiao-fang,GUAN Su-min,et al.Department of Oral Biology,School of Stamatology,The Fourth Military Medical University,Xi'an 710032 |
| |
| Abstract: | Objective:To investigate the role of combination exogenous wild-type PTEN gene with doxycycline on in vitro adhesion,migration and invasion of human mucoepidermoid carcinoma cell line.Methods:The wild-type PTEN tumor suppressor gene or empty vector was introduced into mucoepidermoid carcinoma cell line in vitro,then the cancer cells were treated with doxycycline at dose of 10mg/L for 3days.Cell adhesion was determined by MTT assay.Cell migration was determined by wound-healing assay.Cell invasion was evalua... |
| |
| Keywords: | PTEN gene Doxycycline Mucoepidermoid carcinoma Adhesion Migration Invasion |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
