大鼠小肠移植后外周血T淋巴细胞亚群的变化

利用免疫荧光染色技术及流式细胞仪对大鼠小肠移植后外周血Ｔ淋巴细胞亚群变化进行连续监测，以探讨细胞免疫功能变化在排斥反应中的意义。结果表明，排斥反应时首先出现ＣＤ４阳性细胞显著增高，随后出现ＣＤ８阳性细胞明显下降及ＣＤ４阳性细胞／ＣＤ８阳性细胞比值增高，在排斥反应后期其比值下降；使用环孢素Ａ作免疫抑制的大鼠Ｔ淋巴细胞各亚群均无显著性变化。本实验证明ＣＤ４阳性细胞及ＣＤ８阳性细胞共同参与了排斥反应，根

Studies on changes of T lymphocytes following small bowel transplantation

Rejection is a major obstacle to successful small bowel transplantation and is closely related with cellular immunity. T lymphocyte subsets in peripheral blood were assayed by immunofluorescence stain and flow cytometry after small bowel transplantation in rats. The results showed that there was an evident increase in CD4 subset percentage during the early stage of rejection. And then, CD8 subset declined and the ratio of CD4 /CD8 elevated significantly There was a decrease in the ratio of CD4 /CD8 in the late stage of rejection. These changes were not discovered in CsA treated group. The present study demonstrate that both CD4 and CD8 cells participate in reJectlon in small bowel transplantation, and the changes of CD4 /CD8 ratio indicate both the state of cellular immunlty and the stage of rejection.