长链非编码RNA FENDRR对宣威肺癌XWLC-05细胞增殖、迁移及侵袭的影响

目的:检测非编码RNA FENDRR长链在宣威肺腺癌XWLC-05株细胞与非小细胞肺癌(NSCLC) A549株细胞中的表达,及其对细胞增殖、迁移及侵袭的影响.方法:实时荧光定量PCR检测FENDRR基因在XWLC-05与A549细胞中的表达;构建过表达pEX-3-FENDRR质粒并转染XWLC-05细胞,慢病毒干扰LV3-FENDRR载体转染A549细胞,实时荧光定量PCR检测转染后XWLC-05和A549细胞内FENDRR的表达;MTS法检测细胞增殖;Transwell小室法检测细胞迁移及侵袭能力的变化.结果:XWLC-05细胞中FENDRR基因表达明显较A549细胞低,成功提高转染pEX-3-FENDRR的XWLC-05细胞和降低转染LV3-FEN-DRR质粒的A549细胞中FENDRR mRNA水平.与对照组相比,过表达FENDRR可明显抑制XWLC-05细胞增殖[(1.23±0.13)vs(1.46±0.25),P＜0.05]并降低其迁移、侵袭能力(P＜0.05);干扰FENDRR表达,能够促进A549细胞增殖[(0.85±0.02) vs (0.77±0.08),P＜ 0.05]、迁移与侵袭能力(P＜0.05).结论:lncRNA FENDRR表达下调与宣威肺腺癌的发生发展相关,其在肿瘤恶性化过程中抑制肿瘤细胞的增殖与转移.

Effect of lncRNA FENDRR on proliferation, migration and invasion of the Xuan-wei lung carcinoma XWLC-05 cell

Objective: To detect expressions of FENDRR long non coding RNA(lncRNA) in the lung adenocarcino-ma XWLC-05 cell line from the Xuanwei area and in the non small cell lung cancer (NSCLC) A549 cell line, and to explore its effect on proliferation, migration and invasion of the cell lines. Methods: Expressions of FENDRR gene in the XWLC-05 and the A549 cells were detected by Real-time quantitative fluorescence PCR. Over-expression plasmid pEX-3-FENDRR and lentiviral vector LV3-FENDRR were structured, then respectively transfected into theXWLC-05 and the A549 cells. After the transfection, expressions of FENDRR in the XWLC-05 and the A549 cells were tested by real-time quantitative fluorescence PCR. MTS assay was used to examine proliferation of the cells and Transwell assay used to test migration and invasion abilities of the cells. Results: Expression of ENDRRgene in the XWLC-05cell was obviously lower than that in the A549 cell. Level of FENDRR mRNA in the XWLC- 05cell transfected with pEX-3-FENDRR was enhanced and, which in the A549 cell transfected with LV3-FENDRR reduced. Comparing with the control group, over-expression of FENDRR could significantly inhibit proliferation of the XWLC-05cell ([1.23±0.13] vs [1.46±0.25], P<0.05), and reduce its abilities of migration and invasion (P< 0.05). Interference of FENDRR expression could promote proliferation ([0.85±0.02] vs [0.77±0.08], P< 0.05), mi- gration and invasion of the A549 cell. Conclusion: Down-regulation of lncRNA FENDRR expression could related with occurrence and development of the uanweilung adenocarcinoma, which could play a role of tumor suppres-sion in the process of tumor malignization and inhibit proliferation and metastasis of the tumor cells.