TRIzol法与磁珠法用于丙型肝炎病毒RNA定量检测的比较

目的 比较不同核酸提取方法TRIzol法与磁珠法对丙型肝炎病毒核酸（HCV RNA）定量检测结果的影响。 方法 收集117例丙型肝炎病毒感染阳性患者的血清及其基因分型信息。分别采用TRIzol法与磁珠法提取患者血清样本的HCV RNA，定量PCR检测提取HCV RNA的病毒载量，比较两种提取方法HCV RNA检测结果的差异性。 结果 定量PCR检测结果显示TRIzol法和磁珠法具有良好的线性相关性：y=0.978x+0.063（R2=0.973）。Bland-Altman统计分析显示TRIzol法的检测平均值略低于磁珠法，但无明显统计学差异（P>0.05）. 基因型分析显示1b、2a、3a和6a 亚型之间无显著性差异（P>0.05）.结论TRIzol提取法的HCV定量检测结果和磁珠法相当，且标本用量更少，成本低廉，可在国内广泛使用，且能更大范围地应用在试剂盒的开发和HCV RNA临床检测中。

Comparison of TRIzol method and magnetic beads method in quantitative detection of hepatitis C virus RNA

Objective To compare the effects of TRIzol and magnetic beads methods on quantitative detection of hepatitis C virus (HCV) RNA. Methods Serum samples and genotype information of 117 patients with positive HCV infection were collected. HCV RNA was extracted from serum samples by TRIzol method and magnetic bead method, respectively. And then the viral load of HCV RNA was detected by quantitative PCR to compare the difference between the two methods. Results qPCR results showed that a good linear correlation existed between TRIzol and magnetic beads methods:y=0.978x+0.063 (R²=0.973). Bland-Altman statistical analysis showed that the average logarithmic value of viral load of HCV RNA extracted by TRIzol method was slightly lower than that of magnetic beads method, without significant difference (P>0.05). There were no significant difference among the genotypes 1a, 1b, 2a, 3a or 6a between the two methods (P>0.05). Conclusion TRIzol method is comparable to magnetic beads method in HCV RNA quantitative detection, with less sample volume and lower cost, indicating that it might be widely used for developing kit and HCV RNA clinical detection in China.