HPLC测定痛风颗粒中绿原酸和阿魏酸的含量

目的：建立痛风颗粒中绿原酸和阿魏酸同时测定的方法。方法：采用高效液相色谱法同时测定绿原酸和阿魏酸的含量。色谱柱：YMCJ’sphere ODS-H80 C18柱（4.6mm×150mm,5μm）。流动相：乙腈-1%冰醋酸梯度。流速：1mL/min。测定波长为324nm。结果：绿原酸与阿魏酸分别在12.5～200μg/mL和5～80μg/mL内呈良好线性关系,r均为0.9999。绿原酸平均回收率为102.48%,阿魏酸平均回收率为96.92%,RSD分别为1.25%和1.74%（n=6）。结论：本方法简便、准确,可用于痛风颗粒的质量控制。

Determination of chlorogenic acid and ferlic acid in Gout Granule by high performance liquid chromatorgraphy

Objective： To establish a method for simultaneous determination of chlorogenic acid and ferlic acid in Gout Granule. Methods： High performance liquid chromatorgraphy （HPLC） was used to determine the content of chlorogenic acid and ferlic acid simultaneously. The two acids were separated on YMC J＇ sphere ODS-H80 C18 column （4.6 mm × 150 mm, 5 μm） and were eluted gradiently in mobile phase （acetonitrile-1% glacial acetic acid）. Flow rate was set at 1 mL/min and wavelength was 324 nm.Results： The linearities were in the range of 12.5 - 200 μg for chlorogenic acid and 5 ~ 80 μg/mL for ferlic acid （r=0.999 9）. The average recovery rate was 102.48% （RSD=1.25%） for chlorogenic acid and 96.92% （RSD=1.74%） for ferlic acid.Conclusion： The method is simple and accurate. It can he used for quality control of Gout Granule.