胆红素对烟雾损伤大鼠肺泡巨噬细胞的保护作用

目的:探讨胆红素对烟雾损伤所致肺泡巨噬细胞(AM)的保护作用,寻求防治肺气肿发生、发展的新途径。方法:将健康Wistar大鼠36只,随机分为正常对照组、肺气肿模型组和胆红素干预组。模型组及干预组采用自制大鼠实验性被动吸烟装置熏烟,连续6个月。干预组在熏烟前予以间接胆红素灌胃;模型组和对照组予以生理盐水灌胃。3组动物自由饮食饮水,6个月后处死动物,取材检测;病理形态学检查;采用支气管肺泡灌洗技术收集AM并纯化培养。观察细胞培养液上清中MDA与MO含量。结果:光镜下对照组大鼠肺组织肺泡分布较均匀;模型组大鼠肺组织肺泡普遍扩张,扩张的肺泡融合成较大的囊腔;胆红素干预组肺组织肺泡部分有扩张;模型组与对照比较MDA、MO含量升高(P<0.05);干预组与模型组比较MDA、NO含量降低(P<0.05)。结论:胆红素对于AM有一定的保护作用。

The protective action of bilirubin on injuried alveolar macrophages of rats by smoke

Objective:To investigate the protective action of bilirubin on alveolar macrophage(AM) of pulmonary emphysema to explore a new method of prevention and cure of pulmonary emphysema.Methods:36 healthy Wister rats were divided into 3 groups at random:normal control group,pulmonary emphysema model group,bilirubin treated group.Rats in model group and treated group were exposed to cigarette smoke in a experimental device,six consecutive months.Rats in treated group were instilled bilirubin before exposed to smoke;rats in mlodle group and control group were instilled the equal amount of physiological saline.All of the rats in three guoups were sacrificed to be tested after six months.Pathology were examined.Collect and culture AM of broncho-alveolar lavage fluid(BALF).The levels of MDA,nitricoxide(NO) in cultural supernatant liquid of AM were determined.Results:In model group,the changes of pathological observation were obvious;in treated group,the changes of all were obviously lighten.Model group vs.control group:the content of MDA,NO were increased(P<0.05);treated group vs.model group:the content of MDA,NO were decreased(P<0.05).Conclusion:bilirubin exerts somewhat protection on AMs.