膀胱移行细胞癌E-钙粘连素基因启动子C/A单核苷酸多态性与肿瘤复发的关系

目的　探讨膀胱移行细胞癌 (TCCB)E 钙粘连素 (CDH1)基因启动子 160处C/A单核苷酸多态性 (SNP)与TCCB复发及低分化的关系。方法　运用聚合酶链反应 限制性片段长度多态性分析 (PCR RFLP)方法检测血液标本中CDH1基因启动子上游 160处C/ASNP。通过定期随访肿瘤患者并进行膀胱镜检查以确定肿瘤是否复发。结果　TCCB中CDH 1基因启动子 160处AA ,AC ,CC基因型之间与TCCB的复发具有统计学意义 (P <0 .0 5 ) ;其中AA与CC基因型之间对TCCB复发的相关性差异有统计学意义 (P <0 .0 5 ) ,而CC与AC基因型之间以及AC与CC基因型之间对TCCB复发的相关性差异无统计学意义 (P >0 .0 5 )。以CC基因型为参照 ,AA基因型和AC基因型TCCB复发的危险性均增高 (OR =4.0 5 66,95 %可信区间为 1.83 2 5～ 8.980 1;OR =1.7849,95 %可信区间为 1.10 91～ 2 .872 5 )。该SNP与复发肿瘤发生低分化无明显相关 (P >0 .0 5 )。结论　CDH1基因启动子 160处SNP对TCCB的复发可能具有重要作用。检测该SNP也可作为一种预测患者术后复发的指标。

Relationship between C/A SNP of the E-cadherin gene promoter and high risk of recurrence of transitional cell carcinoma of the bladder

Objective To study the relationship between a 160 C/A single nucleotide polymorphism (SNP) of E cadherin (CDH1) gene promoter and relapse of transitional cell carcinoma of the bladder (TCCB).Methods Patients' genotypes for the C/A SNP were determined by analyzing blood DNA of TCCB patients using polymerase chain reaction restriction fragment length polymorphism (PCR RFLP).Recurrence of TCCB was determined by follow up of cystoscopy and subsequent pathological diagnosis of biopsies.Results There were statistically significant differences in relapse rate of TCCB among three genotypes at 160 position of CDH1 gene promoter ( P < 0.05), especially,the differences in relapse rate between AA genotype and CC genetype was significant ( P < 0.05). Patients with AA genotype and AC genotype had a 4.0 fold and a 1.7 fold higher risk of recurrence of TCCB after initial treatment than those with CC genotype (OR= 4.0566, 95 % CI 1.8325 8.9801; OR= 1.7849, 95 % CI 1.1091 2.8725), respectively.For patients with relapse of TCCB,there was no statistical difference among three genotypes in low differentiation of TCCB ( P > 0.05). Conclusion The SNP at 160 position of CDH1 gene promoter may play an important role in relapse of TCCB.It could serve as a prognostic marker for TCCB.