脱乙酰壳聚糖对大鼠胃溃疡的抑制和修复作用

目的：采用胃溃疡动物模型探讨脱乙酰壳聚糖对胃溃疡的抑制及修复作用。方法：Wistar大鼠分别建立束水应激型（40只）、乙酸烧伤型（32只）和胃黏膜损伤型（32只）胃溃疡模型，胃溃疡模型动物随机分为对照组（0.5% CMC 10 mL^-1•kg^-1）与实验组（脱乙酰壳聚糖Ⅰ组：100 g•L^-1•kg^-1；脱乙酰壳聚糖Ⅱ组：50 g•L^-1•kg^-1；雷尼替丁组：3 g•L^-1•kg^-1），按上述药物剂量灌胃给药，观察溃疡指数和溃疡面积。结果：束水应激型溃疡大鼠溃疡指数脱乙酰壳聚糖Ⅰ组、Ⅱ组和雷尼替丁组低于对照组（P<0.01），各实验组间差异无显著性; 乙酸所致烧灼型胃溃疡和胃黏膜损伤型溃疡大鼠溃疡面积脱乙酰壳聚糖Ⅰ组、Ⅱ组和雷尼替丁组小于对照组（P<0.01）, 各实验组间差异无显著性。结论：脱乙酰壳聚糖对束水应激型溃疡和乙酸烧伤型溃疡有抑制作用，对黏膜损伤型溃疡有修复作用。

Inhibitory and repairing effects of deacetylchitosan on gastric ulcer in rats

Objective To study the inhibitory and repairing effects of deacetylchitosan on gastric ulcer in animal models. Methods Wistar rats were used to set up water-binded stress (n=40), acetic acid-cauterizated (n=32),and gastric mucosa trauma (n=32) models. The rat models were randomly divided into control group (0.5% CMC 10 mL·kg -1) and experimental groups (Deacetylchitosan Ⅰ: 100 g·L -1·kg -1;Deacetylchitosan Ⅱ: 50 g·L -1·kg -1;Ranitidine:3 g·L -1·kg -1). The ulcer indexes and ulcer areas were observed.Results The ulcer indexes in water-binded stress ulcer in Deacetylchitosan Ⅰ, Ⅱ groups and Ranitidine group were lower than that in control (P<0.01),there were no significant differences between experimental groups; The ulcer areas in acetic acid-cauterizated and gastric mucosa trauma ulcer in Deacetylchitosan Ⅰ,Ⅱ groups and Ranitidine group were lower than that in control(P<0.01), there were no significant differences between experimental groups. Conclusion Deacetylchitosan has inhibitory effects on water-binded stress and acetic acid-cauterizated ulcer, and has repairing effect on gastric mucosa trauma ulcer.