须癣毛癣菌表型的研究

目的：采用传统方法对须癣毛癣菌进行表型的分型。方法：采用沙氏琼脂试管培养基（SDA）培养。菌株鉴定及分型依据菌落的形态，菌丝及大小分生孢子的特征，尿素酶试验，葡萄糖米粉试验和体外毛发穿孔试验。结果：36株须癣毛癣菌共分为5个表型，羊毛状、紧密状和绒毛状居多，三型占72．22％，颗粒状最少。螺旋菌丝主要见于绒毛状和粉末状；羊毛和紧密状的镜下结构相似，见大量细长菌丝及少量圆形小分生孢子；颗粒状的大分生孢子最多。结论：须癣毛癣菌分为5个表型，各型镜下结构不同。

Study on the phenotyping of Trichophyton mentagrophytes

Objective: To subtype T. mentagrop hy tes with conventional method. Methods: All the strai ns were cultured on Sabouraud's dextrose agar slopes. Phenotyping of T.m entagrophytes was based on colonial morphology and structure under micro scope, and identification by urease test, glucose rice flour agarose test and ha ir perforation test in vitro. Results:Thir ty-six clinical isolates were classified into 5 phenotypes according to the colo nial morphology and structure under microscope: cottony, powdery, persicolor, gr anular and ulotrichy types. Among these, the ulotrichy, cottony and persicolor p revailed, accounting for 72.22%. The granular was rare. Spiral hyphae more often presented in cottony and powdery types. The microscopic features of ulotrichy a nd persicolor types were similar, with many elongated hyphae and microconidia. M acroconidia were the most in the granular type. Conclusion: Five subtypes of T. mentagrophytes are observed based on the colonial morphology and structure under microscope.