电针对侧脑室注射孤啡肽实验性RA痛阈和皮肤FOS表达的影响 |
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引用本文: | 许建阳,王发强,刘庆安,崔云龙,王海燕,刘志诚,马骋,沈梅红,韩笑,胡一冰.电针对侧脑室注射孤啡肽实验性RA痛阈和皮肤FOS表达的影响[J].针刺研究,2001,26(3):220-221. |
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作者姓名: | 许建阳 王发强 刘庆安 崔云龙 王海燕 刘志诚 马骋 沈梅红 韩笑 胡一冰 |
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作者单位: | 1. 武警总医院,北京,100039 2. 贵阳中医学院99级研究生 3. 南京中医药大学 |
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摘 要: | 目的:观察电针对大鼠实验性关节炎痛阈和皮肤c-fos表达的作用及侧脑室注射孤啡肽(OFQ)对电针效应的影响。方法:将福氏完全佐剂(FCA)注入SD大鼠踝关节腔造成类风湿性关节炎的模型。44只SD大鼠随机分为A模型+侧脑室注射生理盐水(NS)+电针,n=8],B(模型+侧脑室注射OFQ+电针,n=8),C(正常对照,n=8),D(模型,n=10)和E(模型+电针,n=10)组。大鼠麻醉(戊巴比妥钠30mg/kg,i.p)后,参照Noble方法定位,将OFQ(20μL,50μg/mL)或NS(20μL)注射侧脑室(30min内注完,保留1min)。右侧“太溪”和“足三里”针刺后连接WQ-10C多用途电子穴位测定治疗仪,用疏密波形,频率20/100Hz交替,电压2-4V,波宽0.2-0.6ms,持续20min,电针每天1次,连续5天。痛阈用气压弹簧棒测定后右掌心皮肤加压时动物的缩腿反应,每天1次,共测5天。取大鼠右后足垫部皮肤及皮下组织,采用免疫组织化学ABC法测定RA模型建立后各组c-fos表达。结果与结论:①电针对实验性RA关节痛具有明显的镇痛效应;②电针明显提高实验性RA大鼠痛阈,具有明显的后效应,注射OFQ对抗电针提高实验性RA大鼠痛阈,而且对抗电针的后效应;③电针抑制实验性RA诱导的FOS表达,注射OFQ对抗电针抑制FOS的表达。
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关 键 词: | 电针 侧脑室注射 孤啡肽 动物实验 痛阈 FOS |
Influence of Intraventricular Injection of Orphanin on Electroacupuncture Analgesia and Cutaneous C-fos Expression in Experimental Arthritis Rats |
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Abstract: | Objective: To observe the effect of electroacupuncture (EA) on pain thresh old and experimental arthritis induced cutaneous c fos expression and the influ ence of intraventricular injection of orphanin (OFQ) on EA analgesia and arthrit is induced c fos expression in rheumatoid arthritis (RA) SD rats. Met hods: 44 SD rats were randomly divided into A RA model + lateral ventri cular injection (LVI) of normal saline 20 μL + EA, n=8, B (model +L VI of OFQ+EA,n=8), C (normal control, n=8), D (RA model , n=10) and E (model+EA, n=10) groups. RA rat model was esta blished by injection of Freund's complete adjuvant into the ankle joint space. A cupoints of "Taixi" (KI 3) and "Zusanli" (ST 36) on the right hindlimb were puncture d and stimulated electrically with WQ 10C Electronic Acupoint Detection and The rapeutic Apparatus by setting the parameters of frequency 2/100 Hz, 2~4 V, wave width 0.2~0.6 ms and stimulating duration 20 min. Expression of c fos pr otein in the rat plantar region was detected with immunohistochemical method. OFQ 20 μL, 50 μg/mL was injected into the lateral ventricle of the rat brain. Results: ① In RA rats, the pain threshold in A, B, D and E groups was lowered significantly, while after EA treatment, the pain threshold values, particularly on the 3rd, 4t h and 5th day, were increased significantly (P<0.01). There were no si gnificant differences between model+ OFQ+EA and RA model groups in the pain thre shold (P>0 .05), suggesting an antagonizing effect of OFQ on EA analgesia. ② In comparison with normal control group, the positive neurons of c fos expression in A (82.5 4±24.64) and B (174.87±38.23), D (168.42±34.75) and E (99.65±25.36) grou ps w ere increased considerably in comparison with group C (0.00±0.00), showing a st riking increase of c fos expression. The c fos positive neuron numbers in A a nd D groups were significant lower than that of B and D groups (P< 0.01), display ing that EA can significantly suppress RA induced c fos expression while this effect of EA is antagonized by intraventricular injection of OFQ. |
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