| 链霉菌L-183发酵条件优化 |
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| 引用本文： | 黄鹏,尤红煜,郑龙,张焕铃,刘福英,王俊霞. 链霉菌L-183发酵条件优化[J]. 河北医科大学学报, 2007, 28(6): 404-408 |
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| 作者姓名： | 黄鹏 尤红煜 郑龙 张焕铃 刘福英 王俊霞 |
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| 作者单位： | 河北医科大学基础医学院分子生物学研究室,河北,石家庄,050017 |
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| 摘 要： | 目的 对链霉菌L-183生产链霉亲和素(streptavidin,SA)的发酵条件进行优化.方法 通过单因子和正交实验,获得SA发酵培养基的最佳配方比.结果 在优化条件下,SA的产量达到151.5 mg/L.与先前报道相比,产量提高36.5%,且成本降低,操作步骤更为简化.结论 链霉菌L-183生产链霉亲和素的发酵条件优化为规模化生产奠定了基础.
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| 关 键 词： | 链霉素科 链酶亲和素 发酵 |
| 文章编号： | 1007-3205（2007）06-0404-05 |
| 修稿时间： | 2007-03-19 |
OPTIMIZATION OF FERMENTATION CONDITIONS FOR STREPTOMYCES L-183 |
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| HUANG Peng,YOU Hong-yu,ZHENG Long,ZHANG Huan-ling,LIU Fu-ying,WANG Jun-xia. OPTIMIZATION OF FERMENTATION CONDITIONS FOR STREPTOMYCES L-183[J]. Journal of Hebei Medical University, 2007, 28(6): 404-408 |
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| Authors: | HUANG Peng YOU Hong-yu ZHENG Long ZHANG Huan-ling LIU Fu-ying WANG Jun-xia |
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| Affiliation: | Department of Laboratory Animal ,the School of Basic Medical Science, Hebei Medical University, Shijiazhuang 050017 ,China |
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| Abstract: | Objective Fermentation conditions for streptavidin L-183 were optimized to obtain mass production of streptavidin(SA).Methods Factors influencing the secreting of SA were studied by a series of single-factor and orthogonal tests.Results The best medium for the fermentation was found,and 151.5 mg SA per liter can be obtained from the fermentatiobroth,which was 36.5% higher than that described in the literature.Conclusion As the results indicated,by utilizing L-183 and the optimized fermentation protocol,production amount of SA was increased and the basis of mass production was set up. |
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| Keywords: | streptomycetaceae streptavidin fermentation |
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