| RP-HPLC法测定复方金蒲片中延胡索乙素的含量 |
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| 引用本文： | 黄明立,黄东萍,蒋贵发,刘宗河. RP-HPLC法测定复方金蒲片中延胡索乙素的含量[J]. 中国中医药信息杂志, 2010, 17(8): 45-46. DOI: 10.3969/j.issn.1005-5304.2010.08.021 |
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| 作者姓名： | 黄明立 黄东萍 蒋贵发 刘宗河 |
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| 作者单位： | 1. 广西医科大学公共卫生学院,广西,南宁,530021
2. 广西肿瘤研究所,广西,南宁,530021 |
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| 摘 要： | 目的建立复方金蒲片中延胡索乙素的含量测定方法。方法采用高效液相色谱法,色谱柱为Hypersil BDS-C18,流动相为甲醇-5%乙酸(40:60),并用浓氨试液调至pH4.7,流速1.0mL/min,柱温为25℃,检测波长280nm。结果延胡索乙素浓度在0.436～2.180μg范围内峰面积与浓度呈良好线性关系,r=0.9997,平均回收率为98.4%,延胡索乙素与其相邻杂质峰的分离度符合要求。结论本方法简便、专属、重复性好,可用于延胡索乙素的定量分析。
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| 关 键 词： | 复方金蒲片 延胡索乙素 高效液相色谱法 含量测定 |
RP-HPLC Determination of Tetrahydropalmatine in Compound Jinpu Tablets |
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| Abstract: | Objective To develop a method for determination of tetrahydropalmatine in Compound JinpuTablets. Methods RP-HPLC method was used with Hypersil BDS-C18 column, the mobile phase wasmethanol-5% acetic acid （40∶60） adjusted pH to 4.7 by ammonia, flow rate was 1.0 mL/min, and columntemperature was 25 ℃, with the detection wavelength at 280 nm. Results The linear correlation wasobserved for the concentration of 0.436～2.180 μg （r =0.999 7）. The average recovery was 98.4%. Resolutionbetween tetrahydropalmatine and other peaks met the requirements. Conclusion The method is convenient,selective and reproducible for quantitative analysis of tetrahydropalmatine. |
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| Keywords: | Compound Jinpu Tablets tetrahydropalmatine RP-HPLC content determination |
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