HPCE TOF—MS和ESI—MS法研究重组人白细胞介素—2的质量 |
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引用本文: | 周国华,张晓丹,罗国安,章杰兵. HPCE TOF—MS和ESI—MS法研究重组人白细胞介素—2的质量[J]. 解放军药学学报, 2003, 19(1): 16-18 |
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作者姓名: | 周国华 张晓丹 罗国安 章杰兵 |
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作者单位: | 1. 中国人民解放军南京军区联勤部药品仪器检验所,江苏,南京,210002 2. 清华大学分析中心,北京,100084 |
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基金项目: | 全军“九·五”医学科研规划面上项目No .98D0 182 0 0 2 -0 4-0 8 |
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摘 要: | 目的:建立测定重组人白细胞介素-2(IL-2)表达的正确性、产物的纯度和均一性的方法。方法:采用了高效毛细管电泳(HPCE)、时间飞行质谱(TOF-MS)和电喷雾质谱法(ESI-MS)。结果:IL-2样品中存在相对分子量约为15745-15795的杂质,其主成分IL-2在表达和纯化过程中被修饰,其相对分子量比理论值增加了143.8%。结论:HPCE、TOF-MS和ESI-MS法有正交互补性,测定结果可相互印证,质谱法是纯度测定的一种重要工具。
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关 键 词: | 高效毛细管电泳 时间飞行质谱 电喷雾质谱法 重组人白细胞介素-2 |
文章编号: | 1008-9926(2003)01-0016-04 |
修稿时间: | 2002-04-08 |
Quality Study of Recombinant Human Interleukin-II with HPCE and TOF-MS and ESI-MS |
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Abstract: | Aim To establish the method for determining the identity,purity,and homogeneity of recombinant human interleukin-2 (IL-2).Methods High performance capillary electrophoresis (HPCE),matrix assisted laser desorption ionization time of flight mass spectrometry (MALIDI TOF-MS) and electrospray ionization mass spectrometry (ESI-MS) were employed.Results Not only were there impurities with the molecular weight from 15 745 to 15 795 in the sample,but also the ingredient IL-2 was modified during the expression and purification.Conclusion The instrumental methods applied in the paper are orthogonal and complementary,and we can not use one method to replace the other. |
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Keywords: | High performance capillary electrophoresis Matrix assisted laser desorption ionization time of flight mass spectrometry Electrospray ionization mass spectrometry Recombinant human interleukin-2 (IL-2) |
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