贯叶金丝桃总黄酮纯化工艺研究

研究碱化法结合大孔树脂法纯化贯叶连翘总黄酮的工艺条件。方法:以静态饱和吸附量、静态解析率为指标对6种大孔树脂进行了筛选;又以总黄酮含量为指标对提取液上柱前的预处理方式、树脂吸附工艺参数进行了优选。结果:D101大孔树脂吸附量、解析率最好;上样液浓度为0.2 g生药/mL;上样速度为2BV/h;洗脱剂为60%乙醇;洗脱速度为3BV/h;洗脱剂用量为5BV/h。结论:优选的贯叶金丝桃总黄酮纯化工艺稳定、简便可行、适合大生产。

Study on Purification Process of Total Flavonids from Hypericum Perforatum

Objective：To study the purification process conditions of total flavonids from Hypericum Perforatum with alkalization method combined with macroporous resin method. Methods ： In static saturated adsorption capacity,the static analytic rate was as an index of 6 kinds of macroporous resin for selection. Total flavonoids content was as index to extract on column pretreatment method, and resin adsorption process parameters were optimized. Results ：The results showed that D101 macroporous resin adsorption was the best. The sample liquid concentration was 0. 2 g/mL and the washing was 60% ethanol. The elution rate was 3 BV/ h. The dosage of eluent was 5 BV/h. Conclusion： All above showed that the optimization of total flavonids from Hypericum Perforaturn purification process is stable, simple and feasible, and suitable for mass production.