HPLC法同时测定大黄、厚朴药对提取物中7个成分的含量

目的:建立同时测定大黄、厚朴药对提取物中7个成分(芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚、和厚朴酚、厚朴酚)含量的HPLC方法。方法:采用Agilent Eclipse XDB-C18(250 mm×4.6 mm,5μm)色谱柱,以甲醇(A)-0.1%磷酸水(B)为流动相,梯度洗脱,0¹5 min,82%A→85%A;1515 min,82%A→85%A;15³5 min,85%A→90%A;3535 min,85%A→90%A;35⁴0 min,90%A→82%A;4040 min,90%A→82%A;40⁴2 min,82%A→82%A,流速1.0 mL·min-1,检测波长为254 nm,柱温30℃。结果:大黄、厚朴药对提取物中7个成分芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚、和厚朴酚、厚朴酚进样量分别在0.0279042 min,82%A→82%A,流速1.0 mL·min-1,检测波长为254 nm,柱温30℃。结果:大黄、厚朴药对提取物中7个成分芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚、和厚朴酚、厚朴酚进样量分别在0.02790⁰.2790μg(r=0.9992),0.061760.2790μg(r=0.9992),0.06176⁰.6176μg(r=0.9991),0.050400.6176μg(r=0.9991),0.05040⁰.5040μg(r=0.9997),0.19950.5040μg(r=0.9997),0.1995¹.995μg(r=0.9992),0.041281.995μg(r=0.9992),0.04128⁰.4128μg(r=0.9993),0.46440.4128μg(r=0.9993),0.4644⁴.644μg(r=0.9994),0.64324.644μg(r=0.9994),0.6432⁶.432μg(r=0.9991)范围内与峰面积呈良好线性关系;平均回收率(n=5)分别为98.5%、99.4%、99.2%、98.5%、99.5%、99.0%、98.4%。结论:该方法准确可靠、重复性好,可用于大黄、厚朴药对提取物的质量控制。

Simultaneous Determination of Seven Ingredients in Combination Extracts of Rhei Radix et Rhizoma and Magnoliae Officinalis by HPLC

Objective .. To develop an HPLC method for determination of 7 indicative components （ aloe - emodin, rhein, emodin,chrysophanol,physcion, honokio, magnolia） in Rhei Radix et Rhizoma and Magnoliae Officinalis. Methods： Agilent Eclipse XDB - C18 （ 250 mm×4.6 mm,5 μm） was adopted ; the mobile phase was methanol （A） - 0.1% phosphoric acid solution （B） with gradient elution （0 - 15 min,82% A→85 % A ; 15 - 35 min, 85 % A→90% A ; 35 - 40 min, 90% A→82% A ;40 - 42 min, 82% A-→82% A） of methanol - water of 0.1% phosphoric acid at a flow rate of 1.0 mL ·min -1 and the detection wavelength was 254 nm; the column temperature was set at 30 ℃. Results ： The content of 7 indicative components in Rhei Radix et Rhizoma and Magnoliae Officinalis was stable. The method had a good linearity in the ranges of 0.02790 - 0.2790 p.g （ r = 0.9992） for aloe - emodin ;0. 06176 - 0.6176μg （ r = 0.9991 ） for rhein ;0. 05040 - 0. 5040 ixg （ r = 0. 9997 ） for emodin ;0. 1995 - 1. 995 ug（ r = 0. 9992 ） for chrysophanol ;0. 04128 - 0. 4128 ug（ r = 0. 9993 ） for physcion ;0. 4644 -- 4. 644μg （ r = 0. 9994 ） for honokio ;0. 6432 6. 432 μg （r = 0. 9991 ） for magnolia. The average recoveries （n = 5 ） were 98.5% ,99.4% ,99.2% , 98.5% , 99.5% , 99. 0%, 98.4%, respectively. Conclusion：This method is dependable, simple and practical and may be used to control quality of Rhei Radix et Rhizoma and Magnoliae Officinalis.