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猫爪草提取物体外抗菌活性研究
引用本文:卞晓霞,;罗跃娥,;王文洁,;闫利华.猫爪草提取物体外抗菌活性研究[J].辽宁中医杂志,2014(9):1945-1946.
作者姓名:卞晓霞  ;罗跃娥  ;王文洁  ;闫利华
作者单位:[1]天津医学高等专科学校,天津300222; [2]中国中医科学院中药研究所,北京100700
基金项目:中国中医科学院自主选题项目(2011xycz-11)
摘    要:目的:对猫爪草提取物进行了体外抗菌实验,测定其抑菌圈直径和最低抑菌浓度值(MIC),探讨其抗菌活性。方法:采用牛津杯法探讨猫爪草提取物对常见的4种细菌的抑制作用,同时采用微量二倍稀释法测定其对不同供试菌株的最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。结果:猫爪草提取物对不同供试菌种均有一定的抑制作用,且随着浓度的升高,其抑菌活性有逐渐增强的趋势。对金黄色葡萄球菌、绿脓杆菌、大肠杆菌、痢疾杆菌的最低抑菌浓度分别为15.6、31.2、31.2、31.2μg/mL。结论:猫爪草提取物具有较强的抗菌活性,作为抗菌药具有一定的利用价值。

关 键 词:猫爪草提取物  抗菌  最低抑菌浓度  抑菌圈

Study on Antifungal Susceptibility of Radix Ranunculus Ternati Extract
Institution:BIAN Xiaoxia, LUO Yuee, WANG Wenjie ,YAN Lihua (1. Tianjin Medical College, Tianjin 300222, China ;2. China Academy of Chinese Medical Sciences, Beijing 100700, China)
Abstract:Objective:Radix ranunculus ternati extract was made in vitro antifungal experiment to measure the inhibition zone diameter and minimum inhibitory concentration values (MIC). Methods:We used Impatiens Oxford cup method to discuss the four common extracts functions while used micro - dilution method for the determination of its different strains MIC values. Results : Radix ranuneulus ternati extract of different species had a certain extent, and with the concentration increasing, the antibacterial activity had a gradually increasing trend. Trichophyton rubrum, Microsporum canis, Epidermophyton floccosum and Candida albicans minimum inhibitory concentrations were 15.6,31.2,31.2,31.2 μg/mL. Conclusion: Radix ranunculus ternati extract on four kinds of fungi had strong inhibitory activity.
Keywords:Radix ranunculus ternati extract  antibacterial activity  minimum inhibitory concentration  inhibition zone
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