黄芪营养生长期茎叶毛蕊异黄酮苷和黄芪甲苷含量测定

目的:测定黄芪2年生和3年生营养生长期茎叶毛蕊异黄酮苷和黄芪甲苷含量。方法:采用HPLC,色谱条件为色谱柱C18(4.6 mm×150 mm,5μm),流动相为乙腈-水,梯度洗脱,流速1.0 mL·min-1,柱温30℃,检测波长分别为250 nm,203 nm。结果:2年生黄芪营养生长期毛蕊异黄酮苷和黄芪甲苷含量变化范围分别为0.1853⁰.2940,0.23370.2940,0.2337⁰.5367 mg·g-1;3年生黄芪营养生长期毛蕊异黄酮苷和黄芪甲苷含量变化范围分别为0.21800.5367 mg·g-1;3年生黄芪营养生长期毛蕊异黄酮苷和黄芪甲苷含量变化范围分别为0.2180⁰.3830,0.27330.3830,0.2733⁰.6150 mg·g-1。结论:毛蕊异黄酮苷和黄芪甲苷含量3年生高于2年生,随生长时间的延长含量也随之增加;本研究也改进了毛蕊异黄酮苷含量测定方法。

Content Determination of Calycosin - 7 - O - B - D - Glycoside and Astragaloside IV in Vegetative Growth Stage of Stems and Leaves of Radix Astragali

Objective：To determine the contents of calycosin-7 -O- β- D- glycoside and astragalosidelV in the vegetative growth stage of 2 - year and 3 - year stems and leaves of Radix Astragali. Methods ： HPLC method, the chromatographic separation was achieved on a Cls column（ SHIMADZU ,4.6 mmx 150 mm ,5txm） using amobile phase made up of acetonitrile（A） and water （B） at a flow rate of 1.0mL · min - 1. The detection wavelength were set as 250 nm and 203 nm, respectively and the column tem- perature 30 ℃. Results ：The contents of calycosin -7 - O - β - D - glycoside and astragaloside IV of 2 - year Radix Astragali were 0. 1853 -0.2940,0. 2337 - 0. 5367 mg · g-1 respectively. The contents of 3 - year Radix Astragali were 0. 2180 - 0. 3830,0. 2733 -0. 6150 mg · g-1 respectively. Conclus/orr：The contents of 3 -year Radix Astragali were more than that of 2- year one, with growing time also increases the contents of calycosin - 7 - 0 - 3 - D - glycoside and astragaloside IV. This study improves the method of content determination of calycosin- 7 -O-β -D- glycoside and astragaloside Ivof Radix Astragali.