IL-2 induces in vivo suppression by CD4(+)CD25(+)Foxp3(+) regulatory T cells |
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Authors: | Brandenburg Susan Takahashi Takeshi de la Rosa Maurus Janke Marko Karsten Gabriele Muzzulini Till Orinska Zane Bulfone-Paus Silvia Scheffold Alexander |
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Institution: | Immunomodulation Group, Deutsches Rheuma-Forschungszentrum Berlin, Berlin, Germany. |
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Abstract: | Interleukin-2 (IL-2) treatment is currently used to enhance T cell-mediated immune responses against tumors or in viral infections. At the same time, IL-2 is essential for the peripheral homeostasis of CD4(+)CD25(+)Foxp3(+ )regulatory T cells (Treg). In our study, we show that IL-2 is also an important activator of Treg suppressive activity in vivo. IL-2 treatment induces Treg expansion as well as IL-10 production and increases their suppressive potential in vitro. Importantly, in vivo application of IL-2 via gene-gun vaccination using IL-2 encoding DNA plasmids (pIL-2) inhibited naive antigen-specific T cell proliferation as well as a Th1-induced delayed type hypersensitivity response. The suppressive effect can be transferred onto naive animals by Treg from IL-2-treated mice and the suppression depends on the synergistic action of IL-10 and TGF-beta. These data highlight that during therapeutic treatment with IL-2 the concomitant activation of Treg may indeed counteract the intended activation of cellular immunity. |
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Keywords: | DNA vaccination IL‐10 Regulatory T cells |
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