| 表没食子儿茶素没食子酸酯抑制人卵巢癌CoC1细胞生长 |
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| 引用本文： | 韩丽娟.表没食子儿茶素没食子酸酯抑制人卵巢癌CoC1细胞生长[J].长治医学院学报,2008,22(5). |
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| 作者姓名： | 韩丽娟 |
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| 作者单位： | 长治医学院附属和平医院生殖遗传科,046000 |
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| 摘 要： | 目的:观察表没食子儿茶素没食子酸酯(EGCG)抑制人卵巢癌(CoC1)细胞系,细胞生长的生物学效应.方法:通过MTT比色法检测EGCG对CoC1细胞生长的影响;应用细胞计数法研究不同浓度EGCG对CoC1细胞生长曲线的影响;采用平皿克隆形成实验法测定不同剂量EGCG对人卵巢癌(CoC1)细胞锚定依赖性生长能力的影响.结果:EGCG显著抑制人卵巢癌CoC1细胞的生长,呈浓度依赖性;CoC1细胞经EGCG作用后,其细胞群体倍增时间明显延长从正常生长的38.02 h,直至97.56 h,细胞增殖周期延长,从而细胞增殖速度减慢;平皿克隆形成实验法显示:EGCG可抑制人卵巢癌细胞CoC1生长,且呈剂量依赖性.结论:EGCG具有抑制人卵巢癌CoC1细胞生长的作用,其机制可能与细胞增殖周期延长相关.
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| 关 键 词： | 卵巢癌 表没食子儿茶素没食子酸酯 细胞增殖周期 |
Inhibition of Proliferation by Epigallocatechin-3-gallate in Ovarian Cancer Cell Line CoC1 |
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| Han lijuan.Inhibition of Proliferation by Epigallocatechin-3-gallate in Ovarian Cancer Cell Line CoC1[J].Journal of Changzhi Medical College,2008,22(5). |
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| Authors: | Han lijuan |
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| Institution: | Han lijuan Department of Reproductive , Genetic,Heping Hospital of Changzhi Medical College |
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| Abstract: | Objective:To investigate the effect of epigallocatechin-3-gallate on Inhibition of Proliferation in Ovarian cancer cell line CoC1.Methods:The growth inhibition of CoC1 cells was measured by MTT assay.Cell couting method was used to test the influence of growth curve in CoC1 cells treated with different concentrations of EGCG.Cell proliferation of CoC1 cells was evaluated by clone formation test.Results:EGCG significantly inhibited the growth of CoC1 cells,in a concentration dependent manner.Cell counting sh... |
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| Keywords: | Ovarian neoplasms Epigallocatechin-3-gallate Cell doubling time |
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