大鼠胚胎卵巢异体异位移植的实验研究

目的研究大鼠胚胎卵巢异体异位无血管皮下移植后的生长、发育及大鼠内分泌功能恢复情况.方法将孕龄为18-20 d的新鲜或冻融大鼠胚胎卵巢植入去势后4周的大鼠颈部皮下,通过阴道脱落细胞的观察和血清雌二醇（E2）、孕酮（P）的测定了解大鼠内分泌恢复情况,并对移植卵巢及自身子宫进行组织学观察.结果新鲜组和冷冻组分别有52.17%和38.01%的大鼠恢复了动情周期,激素水平分别在移植35 d和49 d恢复到正常水平,移植卵巢明显增大,内可见不同阶段的发育卵泡及黄体,子宫内膜有雌激素效应.结论大鼠胚胎卵巢异体异位无血管皮下移植后能存活、生长发育并具有与正常卵巢相似的内分泌功能.超速冷冻法能较好的保存胚胎卵巢的活性,复苏后与新鲜胚胎卵巢组织具有相同的生理活性.

Experimental Study on Ectopic Allotransplantation of Rats Fetal Ovaries

Objective To study the survival,growth and function restoration of fetal ovary allotransplantation.Methods 18~20 days fresh and frozen-thawed rat ovaries were ectopic allotransplanted subcutaneously to adult female SD rats that had been castrated for 4 weeks.Vaginal smears were taken daily after transplantation to observe the restoration of estrous cycles,and serum oestradiol(E_2) and progesterone(P) levels were measured at different time.Histological morphology of endometrium and ovaries were compared among four groups.Results 52.17%of the recipients in fresh group and 38.01% in frozen-thawed group resulted in restoration of regular estrous cyclic activity,and hormone level became normal on day 35 and day 49 after transplantation.The grafts grew up and follicles in different developing stage and corpora lutea could be observed under microscope.Endometrium had hormone effects.Conclusions This study indicates that subcutaneously allotransplanted fetal ovaries can survive,develop and restore the function of secreting sex hormone.The ultraspeed cooling rats fetal ovaries can be survival when they were thawed and develop and secrete hormone as fresh ovaries after being allotransplanted.