肝细胞生成素及肝部分切除诱导肝再生基因PC3的表达

目的 观察重组人肝细胞生成素（rhHPO)和肝部分切除迅速诱导瞬时早期反应基因表达情况。方法 利用表达性差异显示分析技术和序列分析研究2／3肝部分切除后1h及原代培养大鼠肝细胞体系的选择性基因表达。结果 揭示EST集中的大部分为瞬时早期反应基因，发现一种可能与肝再生调控相关的新基因PC3，Northern杂交证实2／3肝部分切除可迅速诱导PC3基因的表达，其表达高峰为术后1－2h。HPO在原代培养大鼠肝细胞体系中可迅速诱导瞬时早期反应基因（c-fos,LRF-1和PC3等）表达。结论 HPO和肝部分切除可迅速诱导瞬时早期反应基因表达，PC3基因是一种与肝再生调控密切相关的早期反应基因。

Rapid induction of mRNAs for liver regeneration genes by hepatopoietin and partial hepatectomy

Objective To investigate the effect of recombinant human hepatopoietin (rhHPO) and partial hepatec-tomy on rapidly induced expression of immediate early gene. Methods We investigated the different gene expression within 1 hour after 2/3 partial hepatectomy by representational difference analysis and in primary cultured hepatocytes system. Results In the expressed sequence tag (EST) library, we identified that most of these genes were immediate early gene, and found one new gene PC3 that might be associated to liver regeneration in the EST library. Moreover, PC3 gene was rapidly induced after 2/3 partial hepatectomy and the expressing peak was within 1-2 hours after operation. HPO can rapidly induce the expression of these genes (c-fos, LRF-1, and PC3, etc.) in primarily cultured rat hepatocyte, which might be one of HPO molecular mechanism on stimulating hepatocyte proliferation. Conclusions rhHPO and partial hepatectomy can rapidly induce the expression of immediate early gene. PC3 gene is immediate early gene related to liver regeneration.