补肾中药对雄激素致不孕大鼠卵巢核仁组成区蛋白的影响

观察补肾中药对雄激素致不孕大鼠卵巢核仁组成区蛋白的影响。用ＳＤ雌性大鼠幼仔，出生９ｄ龄注射丙酸睾丸酮，制成雄激素致不孕大鼠（ＡＳＲ）模型。８０ｄ龄灌服补肾中药水溶液两周。１００ｄ龄心脏灌注处死。以核仁组成区蛋白嗜银染色（ＡｇＮＯＲ）和增殖细胞核杭原（ＰｃＮＡ）为指标，观察卵巢颗粒细胞、间质腺细胞的形态学变化。结果：补肾中药能够使ＡＳＲ卵巢颗粒细胞增殖、卵泡发育。治疗组的ＡｇＮＯＲ和ＰｃＮＡ计数明显高于模型组，而与对照组之间无显著差异。结论：补肾中药的这种作用可能是通过调节了下丘脑－垂体－卵巢性腺功能的结果，从而促使卵巢颗粒细胞发育、卵泡成熟。

OVARY NUCLEAR PROTEIN DETECTION IN ANDROGEN STERILIZED RATS TREATED WITH KIDNEY TONIFYING HERBS

PURPOSE To observe the effect of ovary unclear protein in androgenized steriled rats treated with kidney tonifying herbs. METHODS Yong rats of 9-day old with testosterone propionate subcutaneously injected were used as an androgen sterilized rats (ASR) model. On 80-day,herbal extract of tonifying the kidney was fed for 14-days,All animals were sacrificed by cardiac perfusion method on 100-day of ages.Morphological studies with argyrophilic nucleolar organiser region (AgNOR) and proliferating cell nuclear antigen (PcNA) were used for observation on the morphological change of ovary granulose cell,interstilial gland cell. RESULTS In A group (treated with testosterone propionate only) AgNOR and PcNA decreased than C group (treated with camellia seed-oil) , A H group (treated with herbs) (P<0.01) . A H group was increased same C group. CONCLUSIONS It is suggest that kidney tonifying herbs regulated hypothalamus-pituitary-ovary axis fuction,as a result ovary granulose cell hyperplasias and follicle development.