合成油的营养评价——(三)在实验性高脂血症大鼠的恢复期中合成油对其体重、肝重、血脂与肝脂水平的影响

在12％猪油饲料中加入胆固醇和胆酸饲养雌性断乳大鼠52日,以诱导动物产生高胆固醇血症。然后根据血清胆固醇水平和体重将大鼠分为4组,其中1组被处死作为负对照,另3组则分别饲以无胆固醇和胆酸的合成油,猪油或豆油饲料3周,观察不同油脂饲料对大鼠恢复的影响。在实验期内,测量体重、记录摄食量、测定粪的类固醇排出量、测定血清胆固醇总量和HDL含量、称量肝重和测定肝胆固醇含量。实验结果如下: (1)在恢复期的3周内,合成油组的动物体重增加和摄食量均显著低于其他两组。用共变数分析方法消除其摄食量的影响后,各组动物的体重增加尤显著性差别。 (2)52日诱导期末,大鼠血清总胆固醇量平均增为450mg/dl。恢复期的第一周即下降了60％,但到第3周时仍未能恢复到正常水平。3个组在任何一周的均值都未现出明显不同。 (3)HDL-C的测定结果指出在3周的恢复期后,猪油组的明显低于其他两组。 (4)动物的肝重和肝脂含量的恢复情况相似。肝胆固醇水平则以猪油组的较高,合成油和豆油组的均值间无显著性差别。 (5)粪的固醇平衡实验结果表明,在恢复期,猪油饲料组动物的粪中性类固醇排出量最低,固醇平衡量最高。 总结上述结果,可以看到在大鼠高胆固醇血症的恢复过程中,饲料油的种类对动物的体重、血清胆固醇、肝重和肝脂含量无

NUTRITIONAL EVALUATION OF SYNTHETIC OIL Ⅲ. Effect of Synthetic Oil on Body Weight,Liver Weight,Blood and Liver Lipids of Rats During Rehabilitation of Hypercholesterolemia

Weanling female rats were fed 12% lard diet supplemented with 2 g cholesterol and 60 mg cholic acid per 100 g diet for 52 days. Blood cholesterol levels were then analyzed for individual rat. Animals were divided into 4 groups according to their blood cholesterol levels and body weights. One group of animals, regarded as negative control, was killed immediately. The other 3 groups were fed 12% level of synthetic oil, lard or soybean oil diet respectively without cholesterol and cholic acid supplementation for 3 weeks. During the 3 weeks of rehabilitation, the following parameters were measured: body weight and serum cholesterol levels, every week; food intakes, every other day; high density lipoprotein cholesterol (HDL-C) levels at the beginning and the end of the rehabilitation period; fecal steroids excretion, including neutral and acid steroids from the 2nd to the 4th day of rehabilitation; liver weights, liver fat contents and liver cholesterol levels after animals were killed.The results showed that no significant effects on body weights, serum cholesterol levels, liver weights and liver fat contents of different kinds of dietary fat were observed during the rehabilitation period of hyperch-olesterolemia. But synthetic oil, as well as soybean oil diet caused a lower liver cholesterol levels, and higher levels of both serum HDL-C and fecal neutral steroids.