氟康唑诱导菌丝相马尔尼菲青霉菌产67.5kDa蛋白

目的探讨在体外加入氟康唑培养菌丝相马尔尼菲青霉菌的外分泌蛋白质变化情况。方法11株马尔尼菲青霉菌分别接种于含8μg/mL和不含氟康唑的30mL沙氏液基(SDB)中,25℃培养1周。E-test法测定不同培养液中菌丝对氟康唑的药敏。同时提取培养液中的蛋白质,聚丙烯酰胺凝胶电泳比较外分泌蛋白质差异。结果含氟康唑SDB中的马尔尼菲青霉菌菌丝悬液均变为玫瑰红色,不含氟康唑SDB中菌丝悬液颜色不变红。含氟康唑SDB中菌丝氟康唑MIC值显著增高(P<0.01),而且分泌67.5kDa蛋白质。结论在25℃沙氏液基中,8μg/mL氟康唑可以诱导马尔尼菲青霉菌产红色色素并分泌67.5kDa蛋白质。

Fluconazole Inducing Penicillium Marneffei Mycelial Form to Produce 67.5-Kilodalton Protein

Objective To investigate the change of exocrine protein during penicillium marneffei mycelial form cultured with fluconazole in vitro.Methods Eleven strains of penicillium marneffei were respectively cultured in 30 mL Sabouraud dextrose broth(SDB) with fluconazole concentration of 8 μg/mL and without fluconazole at 25℃ for one week.The MIC of fluconazole against mycelial fungi in different liquid culture were tested by E-test method.The proteins in liquid culture were extracted and secreted products were analyzed by polyacrylamide gel electrophoresis.Results The suspension of mycelial penicillium marneffei cultured in SDB with fluconazole presented rose color,but the suspension of mycelia cultured in SDB without fluconazole not presented rose color.The MIC of fluconazole against mycelia cultured with fluconazole increased significantly(P<0.01) and furthermore they secreted 67.5-kilodalton protein.Conclusion Fluconazole concentration of 8 μg/mL can induce penicillium marneffei to secret red pigment and 67.5-kilodalton protein in SDB at 25℃.