nm23-H1基因转染对肺癌L9981细胞黏附分子表达的影响

目的:探讨nm23-H1基因转染对肺癌L9981细胞黏附分子表达的影响。方法:利用细胞黏附实验、Boyden小室法研究转染前后肺癌L9981细胞黏附、侵袭能力的改变。分别利用逆转录-PCR(RT-PCR)和流式细胞术检测转染前后E-cadherin、integrin β1和integrin β3 mRNA和蛋白的表达。结果:转染后L9981细胞株黏附性以及侵袭性均降低。与转染空载体组相比,转染nm23-H1质粒后L9981细胞E-cadherin mRNA和蛋白表达均增强,integrin β1和integrin β3 mRNA和蛋白表达均减弱,差异均具有统计学意义(P均<0.05)。结论:nm23-H1基因具有逆转肺癌恶性转移表型的能力,其对肺癌L9981细胞株E-cadherin表达具有正调控作用,对integrin β1和integrin β3表达具有负调控作用。

Influences of nm23-H1 gene transfection on expressions of adhesion molecules in lung cancer cell line L9981

OBJECTIVE:To study influences of nm23-H1 gene transfection on the expressions of adhesion molecules in lung cancer cell line L9981. METHODS:Cell adhesion experiment and Boyden room were used to evaluate the change of cell adhesion and invasion. Semi-RT-PCR and flow cytometry were used to detect the mRNA and protein expressions of E-cadherin, integrin β1 and integrin β3 betwteen transfected and non-transfected cell lines. RESULTS: nm23-H1 cell line in vitro adhesion and invasion were all down-regulated. mRNA of E-cadherin in the transfected cell line was stronger than that of non-transfected cell line, but mRNAs of integrin β1 and integrin β3 of transfected cell line were weaker than that of non-transfected cell line. Flow cytometry showed similar results as above. CONCLUSION: nm23-H1 could reverse lung cancer malignant phenotype. nm23-H1 could up-regulate E-cadherin expression, while down-regulate expressions of integrins β1 and β3, thus inhibit the metastasis of lung cancer cells.