| 测定红车轴草中刺芒柄花素含量的新方法 |
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| 引用本文： | 周广为,慕慧,李岳锋,杨芸. 测定红车轴草中刺芒柄花素含量的新方法[J]. 中药材, 2007, 30(1): 54-56 |
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| 作者姓名： | 周广为 慕慧 李岳锋 杨芸 |
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| 作者单位： | 西安交通大学理学院化学系,陕西西安,710049 |
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| 摘 要： | 目的:建立了一种用同步荧光测定红车轴草中刺芒柄花素含量的新方法。方法:研究了检测条件对刺芒柄花素荧光强度的影响,以及实验的准确性、重现性和回收率。结果:该方法的线性回归方程为y=1440x-0.263,r=0.999,检出限为1.50×10-9mol/L。本方法测得红车轴草中刺芒柄花素的含量与HPLC法的结果相对偏差为1.65%。结论:说明本方法结果准确,可以满足科研和生产的要求。
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| 关 键 词： | 荧光分析法 红车轴草 刺芒柄花素 |
| 文章编号： | 1001-4454(2007)01-0054-03 |
| 修稿时间： | 2006-07-05 |
A New Method in Determination of Formononetin Content in Trifolium pratense |
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| ZHOU Guang-wei,MU Hui,LI Yue-feng,YANG Yun. A New Method in Determination of Formononetin Content in Trifolium pratense[J]. Journal of Chinese medicinal materials, 2007, 30(1): 54-56 |
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| Authors: | ZHOU Guang-wei MU Hui LI Yue-feng YANG Yun |
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| Affiliation: | Department of Chemistry, Xián Jiaotong University, Xián 710049, China. |
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| Abstract: | OBJECTIVE: A new method was established to determine the formononetin content in Trifoblium pratense L. METHODS: Effects of fluorescence intensity, as well as its precision, repetition and recovery efficency were studied. RESULTS: The linear relationship was y = 1440 x -0.263, r = 0.999, the detection limit was 1.50 x 10(-9) mol/L, the relative deviation between this method and HPLC method was 1.65%. CONCLUSION: The study indicated that this method was viable, and could be used in both scientific research and industrial production. |
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| Keywords: | Fluorometry Trifolium pratense L. Formononetin |
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