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| GnRH类似物对化疗大鼠卵泡凋亡的影响 | |
| 引用本文： | 彭萍,莫亚勤,杨传红,张伟,张艳玲,刘佳,叶长烂,杨冬梓. GnRH类似物对化疗大鼠卵泡凋亡的影响[J]. 南方医科大学学报, 2010, 30(1): 106 |
| 作者姓名： | 彭萍 莫亚勤 杨传红 张伟 张艳玲 刘佳 叶长烂 杨冬梓 |
| 作者单位： | 1. 中山大学孙逸仙纪念医院妇产科,广东广州510120;广州军区广州总医院妇产科,广东广州510010 2. 中山大学孙逸仙纪念医院妇产科,广东广州,510120 3. 广州军区广州总医院妇产科,广东广州,510010 |
| 基金项目： | 广东省科技计划项目(2007B080701014);;广东省医学科学技术研究基金(A475);;广东省科技计划项目(2008B080701026);;广东省自然科学基金博士启动项目(8451001002001274);;中山大学“临床医学研究5010计划”项目(2007017) |
| 摘 要： | 目的探讨促性腺激素释放激素激动剂(GnRH-a)、拮抗剂(GnRH-ant)对环磷酰胺(CTX)诱导的大鼠卵泡凋亡的干预作用。方法36只雌性Sprague-Dawley大鼠随机分为6组,即实验对照组、CTX组、GnRH-a+生理盐水(NS)组、GnRH-a+CTX组、GnRH-ant+NS组及GnRH-ant+CTX组,每组6只,于结束用药的第1～2周内的动情间期处死,采用末端转移酶介导的缺口末端标记法(TUNEL)结合透射电镜检测卵泡的凋亡情况。结果(1)TUNEL检测晚期生长卵泡的颗粒细胞凋亡率高于早期卵泡,使用CTX组别的卵母细胞、颗粒细胞的凋亡率高于相应对照组,其差异均有显著性意义(P<0.05)。其中GnRH-a两组[(33.40±4.59)～(73.25±5.35)]的颗粒细胞凋亡率较GnRH-ant两组[(27.46±4.52)～(49.38±5.02)]明显升高,其差异有显著性意义(P<0.05);但早期卵泡卵母细胞的凋亡率在GnRH-a两组[(23.48±4.25)～(36.15±4.23)]与GnRH-ant两组[(21.47±3.81)～(34.04±5.54)]的相应组间差异无显著性意... |
| 关 键 词： | 促性腺激素释放激素类似物 卵泡 细胞凋亡 |
Effects of gonadotroph-releasing hormone analogues on follicle apoptosis in rats with chemotherapy-induced ovarian damage |
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| PENG Ping,MO Yaqin,YANG Chuan-hong,ZHANG Wei,ZHENG Yan-ling,LIU Jia,YE Chang-lan,YANG Dong-zi. Effects of gonadotroph-releasing hormone analogues on follicle apoptosis in rats with chemotherapy-induced ovarian damage[J]. Journal of Southern Medical University, 2010, 30(1): 106 | |
| Authors: | PENG Ping MO Yaqin YANG Chuan-hong ZHANG Wei ZHENG Yan-ling LIU Jia YE Chang-lan YANG Dong-zi |
| Affiliation: | PENG Ping1,2,MO Ya-qin1,YANG Chuan-hong2,ZHANG Wei2,ZHENG Yan-ling2,LIU Jia2,YE Chang-lan2,YANG Dong-zi1 1Department ofObstetrics, Gynecology,Sun Yat-sen Memorial Hospital,Sun Yat-sen University,Guangzhou 510120,China,2Department of Obstetrics , Gynecology,General Hospital,Guangzhou Military Area,Guangzhou 510010 |
| Abstract: | Objective To study the effects of gonadotroph-releasing hormone (GnRH) agonist (GnRH-a) and GnRH antagonist (GnRH-ant) on cyclophosphamide (CTX)-induced follicle apoptosis in female rats. Methods Thirty-six female Sprague- Dawley rats were randomized into 6 groups, namely normal saline (NS), CTX, GnRH-a+NS, GnRH-a+CTX, GnRH-ant+NS, and GnRH-ant+CTX groups. The rats were sacrificed between the first and second week after the treatments., and the follicle apoptosis was investigated using TUNEL assay and trans... |
| Keywords: | gonadotroph-releasing hormone analogue follicles cell apoptosis |
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