| 蜜蜂毒中蜂毒素的分离纯化方法及含量测定 |
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| 引用本文： | 刘岭,凌昌全,胡晋红,黄雪强. 蜜蜂毒中蜂毒素的分离纯化方法及含量测定[J]. 第二军医大学学报, 2001, 22(7): 609-611 |
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| 作者姓名： | 刘岭 凌昌全 胡晋红 黄雪强 |
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| 作者单位： | 1. 第二军医大学长海医院中医科,
2. 长海医院药学部 |
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| 基金项目： | 军队“九五”重点课题 ( 98DJ-3 2 69) |
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| 摘 要： | 目的：从蜜蜂毒中分离纯化蜂毒素（melittin)，建立以SDS-聚丙烯酰 胺凝胶电泳(SDS－PAGE）及HPLC进行蜂毒素定性定量分析的方法。方法：用Sephadex G-25、Sephadex G-50、Sephadex G-75三步层析法从蜜蜂毒中分离纯化蜂毒素，以SDS－PAGE定性，以HPLC进行含量测定。结果：样品经SDS－PAGE测定为单一条带，相对分子质量约3000。HPLC含量测定，平均回收率为95．97％，相对标准差（RSD）为1．07％。结论：以上述分离纯化法可制得高纯度的蜂毒素，检测方法准确、可靠、简单。
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| 关 键 词： | 蜂毒素 色谱法 凝胶 聚丙烯酰胺凝胶 高压液相 SDS－PAGE HPLC |
| 文章编号： | 0258-879(2001)07-0609-03 |
| 修稿时间： | 2000-12-11 |
Isolation, purification and content determination of melittin from bee venom |
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| LIU Ling ,LING Chang Quan ,HU Jin Hong ,HUANG Xue Qiang. Isolation, purification and content determination of melittin from bee venom[J]. Former Academic Journal of Second Military Medical University, 2001, 22(7): 609-611 |
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| Authors: | LIU Ling LING Chang Quan HU Jin Hong HUANG Xue Qiang |
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| Affiliation: | LIU Ling 1,LING Chang Quan 1,HU Jin Hong 2,HUANG Xue Qiang 1 |
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| Abstract: | Objective:To isolate and purify melittin from bee venom and to establish an SDS PAGE and HPLC method for the determination of melittin content.Methods:Melittin was isolated and purified from bee venom by gel filtration on Sephadex G 25,Sephadex G 50 and Sephadex G 75.Homogenicity and molecular weight of melittin were determined by SDS PAGE and HPLC.Results:The sample was a single peptide chain with a molecular mass of about 3 000 dalton as revealed by SDS PAGE.The average recovery was 95.97% and RSD was 1.07%.Conclusion:Melittin prepared by a combination of gel filtration has high purity.The method for the determination of melittin with SDS PAGE and HPLC is accurate,reliable and simple. [ |
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| Keywords: | melittin chromatography gel electrophoresis polyacrylamidegel chromatography high pressure liquid |
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