肺癌患者柯萨奇病毒和腺病毒受体的表达及其临床意义

  目的   探索柯萨奇病毒和腺病毒受体（Coxsackie and adenovirus receptor，CAR）与肺癌的关系，为使用基于腺病毒载体的基因治疗方案提供依据。   方法   运用免疫组织化学法检测112例肺癌患者肿瘤组织、癌旁组织以及正常组织中CAR的表达；通过RT-PCR和Western blot法检测32例患者CAR mRNA及CAR蛋白的表达。   结果   在正常肺组织中未见CAR表达，癌旁组织中可见少量的CAR表达。鳞状细胞癌和腺癌中CAR阳性表达比率分别为43%和70%，均较癌旁组织明显升高。同时，腺癌中CAR的表达也显著高于鳞状细胞癌，RT-PCR和Western blot法进一步证实免疫组织化学法检测结果。   结论   肺癌中CAR过表达，在腺癌中尤为明显，提示CAR可能参与了肺癌的发生发展，这可能对基因治疗肺癌的研究提供了较好的理论基础。 

Expression of Coxsackie and Adenoviruses Receptors and Its Clinical Significance in Human Lung Cancer

  Objective   To investigate the expression of coxsackie and adenovirus receptors (CAR) and the progression of lung cancer to provide a foundation for the clinical treatment of lung cancer using adenovirus vector-based gene therapy.   Methods   The CAR expression in lung cancer, paraneoplastic, and normal tissues of 112 patients was assayed by immunohistochemistry (IHC). The mRNA and protein expression of CAR in 32 cases were determined by RT-PCR and Western blot, respectively. All data were analyzed using SPSS16.0, and a Kaplan-Meier survival curve was constructed. A log-rank test was also conducted.   Results   No CAR expression was found in normal lung tissues. CAR expression was higher in the paraneoplastic tissues than in the normal lung tissues. Furthermore, CAR expression was higher in adenocarcinoma (70%) than in the squamous cell cancer (43%). However, both were significantly higher compared with that in the paraneoplastic tissues (P < 0.01). The results of the mRNA expression by RT-PCR and protein expression by Western blot agree with the results obtained from IHC.   Conclusion   CAR is overexpressed in the human lung cancer, especially in adenocarcinoma, which suggests its function in the occurrence and development of lung cancer. CAR participates in the pathogenic or developmental process in lung cancer. Our research provides reliable basis for adenovirus-mediated gene therapy of lung cancer.