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| 头穴丛刺法对脑缺血再灌注大鼠脑组织AQP_4表达的影响 | |
| 引用本文： | 刘婷婷,朱文增,金弘,黄日龙.头穴丛刺法对脑缺血再灌注大鼠脑组织AQP_4表达的影响[J].针灸临床杂志,2012,28(8):55-58. |
| 作者姓名： | 刘婷婷 朱文增 金弘 黄日龙 |
| 作者单位： | 1. 黑龙江中医药大学,黑龙江哈尔滨,150040 2. 中国中医科学院广安门医院,北京,100053 3. 黑龙江中医药大学附属第一医院,黑龙江哈尔滨,150040 4. 安徽省中医院,安徽合肥,230031 |
| 基金项目： | 黑龙江省自然科学基金项目,编号:2006D-61 |
| 摘 要： | 目的：研究头穴丛刺法对脑缺血再灌注大鼠的脑保护作用机制。方法：采用改良的线栓法制备大鼠大脑中动脉缺血再灌注模型。将Wistar雄性大鼠,随机分成3组：假手术组、模型组、头穴丛刺组。各组随机分成6 h、1 d、2 d、3 d共5个时间点,采用免疫组织化学技术检测大鼠脑缺血再灌注后AQP4蛋白表达变化及头穴丛刺法治疗对它们的影响。结果：免疫组化结果显示与模型组比较,头穴丛刺组损伤侧脑组织AQP4蛋白表达水平明显升高（P〈0.05或P〈0.01）。结论：头穴丛刺法能够调节AQP4的表达,减轻由于再灌注损伤导致的血脑屏障通透性增强,从而减轻脑水肿,在脑缺血再灌注早期起到挽救和保护受损神经元、改善神经功能的治疗作用。 |
| 关 键 词： | 脑缺血再灌注 头穴丛刺法 脑水肿 水通道蛋白-4 |
The Effect of Cluster Acupuncture on Scalp Points on AQP4 Expression in Cerebral Ischemica Reperfusion Rats |
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| LIU Ting-ting , ZHU Wen-zeng , JIN Hong , HUANG Ri-long.The Effect of Cluster Acupuncture on Scalp Points on AQP4 Expression in Cerebral Ischemica Reperfusion Rats[J].Journal of Clinical Acupuncture and Moxibustion,2012,28(8):55-58. | |
| Authors: | LIU Ting-ting ZHU Wen-zeng JIN Hong HUANG Ri-long |
| Institution: | 1.Heilongjiang University of Chinese Medicine,Harbin 150040,China; 2.Guang’ anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China; 3.The First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150040,China; 4.Anhui Provincial Hospital of Chinese Medicine,Hefei 230031,China) |
| Abstract: | Objective:To explore cerebral protection mechanism of cluster acupuncture on scalp points(CASP) in cerebral ischemia reperfusion(CIR) rats.Methods:Focal cerebral ischemia was induced by intraluminal middle cerebral artery occlusion(MCAO) method using a monofilament thread in rats.Male Wistar rats were divided randomly into three groups: sham group、model group、and CASP group;each group was separated into 6 h,l d,2 d,3 d,5 d after reperfusion as subgroup for investigation.The expression of AQP4 protein in CIR rats were measured by immunohistochemical method and the influence of the expression by CASP was observed.Results:Immunohistochemistry results indicated that compared with model group,the expression of AQP4 has been significantly increased in CASP group(P<0.05,P<0.01).Conclusion:CASP method can regulate AQP4 expression to reduce BBB permeability to prevent cerebral edema,through reducing cerebral edema,CASP method can save and protect damaged neurons,improve nervous function at early stage of CIR. |
| Keywords: | Cerebral ischemia reperfusion Cluster acupuncture on scalp points(CASP) Cerebral edema AQP4 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |