| 维甲酸和联合应用神经营养因子对新生大鼠神经干细胞分化的影响 |
| |
| 引用本文： | 高虹,龚珊,单立冬,萨喆燕,蒋星红. 维甲酸和联合应用神经营养因子对新生大鼠神经干细胞分化的影响[J]. 苏州大学学报(自然科学版), 2008, 28(4) |
| |
| 作者姓名： | 高虹 龚珊 单立冬 萨喆燕 蒋星红 |
| |
| 作者单位： | 苏州大学医学部神经生物学教研室,衰老与神经疾病实验室,江苏苏州215123 |
| |
| 摘 要： | 目的研究全反式维甲酸（ATRA）及联合应用神经营养因子（BDNF，GDNF）对体外培养的神经干细胞（NSCs）分化的影响。方法取新生SD大鼠的前脑室下带（SVZ）区，按NSCs的常规培养方法分离、培养。用免疫细胞化学法鉴定巢蛋白（nestin）、微管相关蛋白-2（MAP-2）、胶质原纤维酸性蛋白（GFAP）的表达，以此来观察ATRA、BDNF、GDNF单独或联合应用对次代神经球细胞分化的作用。结果原代及次代神经球均显示nestin阳性，并可分化为MAP-2阳性神经元样细胞及GFAP阳性胶质细胞样细胞。1μmol／LATRA可促进NSCs分化为MAP-2阳性细胞的比例达（29．14±5．00）％，显著高于对照组的（7．19±1．21）％，差异有高度统计学意义（P〈0．001）。ATRA联合应用10ng／ml的BDNF或GDNF，与单独使用ATRA比较，并不显著提高NSCs分化为MAP-2阳性细胞的比例。结论ATRA可促进神经干细胞向神经元方向分化，ATRA联合应用BDNF或GDNF无明显的协同作用。
|
| 关 键 词： | 神经干细胞 全反式维甲酸 神经营养因子 分化 BDNF GDNF 培养 |
Effects of Retinoic Acid Alone and Combined with Neurotrophic Factors on Differentiation of Neural Stem Cells from Neonatal Rats |
| |
| GAO Hong,GONG Shan,SHAN Li-dong,SA Zhe-yan,JIANG Xing-hong. Effects of Retinoic Acid Alone and Combined with Neurotrophic Factors on Differentiation of Neural Stem Cells from Neonatal Rats[J]. Suzhou University Journal of Medical Science, 2008, 28(4) |
| |
| Authors: | GAO Hong GONG Shan SHAN Li-dong SA Zhe-yan JIANG Xing-hong |
| |
| Affiliation: | GAO Hong,GONG Shan,SHAN Li-dong,SA Zhe-yan,JIANG Xing-hong(Dept of Neurobiology,Lab of Aging , Nervous Diseases,Medical College,Suzhou University,Jiangsu Suzhou 215123,China) |
| |
| Abstract: | Objective To explore the effects of all trans-retinoic acid(ATRA) alone or combined with neurotrophic factors(BDNF,GDNF) on the differentiation of neural stem cells(NSCs) from neonatal rats.Methods The forebrain subventricular zone(SVZ) region from neonatal SD rats was dispersed and cultivated.The secondary neurospheres were used to observe the effects of ATRA,BDNF or GDNF alone and ATRA combined with BDNF or GDNF on the differentiation of NSCs.Immunocytochemistry was used to observe the expression of cell ... |
| |
| Keywords: | BDNF GDNF |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
