CC10在苦参碱诱导的肺腺癌细胞凋亡中的表达

目的 探讨Clara细胞分泌蛋白10-kDa（CC10）在苦参碱诱导的肺腺癌细胞凋亡中的表达。方法 肺腺癌A549细胞进行传代培养72h后，分别加入30、60、120、240、480mg/L的苦参碱，MTT法检测其对A549细胞生长的抑制作用．流式细胞仪检测细胞凋亡．Westcrn blot及RT-PCR检测CC10蛋白及mRNA的表达。结果 不同浓度的苦参碱对肺腺癌细胞生长均有抑制作用．并呈浓度依赖性。实验组细胞凋亡率和CC10基因表达率高于对照组，也呈浓度依赖性。结论 CC10基因表达增高可能在苦参碱诱导肺腺癌A319细胞凋亡中起重要作用。

Expression of CC10 Gene in the Matrine-induced Apoptosis of Lung Adenocarcinoma Cell Line

Objective To study the expression of Clara cell secretory protein 10-kDa(CC10) in the matrine-induced apoptosis of lung adenocarcinoma.Methods After lung adenocarcinoma A549 cells were cultured for 72 h,matrine of 30,60,120,240 and 480 mg/L was added separately.The inhibitory effects of matrine on the growth of A549 cells were tested by using MTT method,apoptosis was detected by flow cytometry,and the expression of CC10 protein and mRNA by Western blot and RT-PCR respectively.Results Matrine with different concentrations had inhibitory effects on the growth of lung adenocarcinoma cells in a concentration-dependent manner.In the experimental group,apoptotic rate and the positive expression rate of CC10 gene were higher than in the control group in a concentration-dependent manner also.Conclusion The overexpression of CC10 might play an important role in the matrine-induced apoptosis of lung adenocarcinoma A549 cells.