| 诺帝对HPV16型亚基因永生化人宫颈上皮细胞的诱导分化作用 |
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| 引用本文： | 赵敬,赵涌,卞修武.诺帝对HPV16型亚基因永生化人宫颈上皮细胞的诱导分化作用[J].第三军医大学学报,2007,29(1):43-46. |
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| 作者姓名： | 赵敬 赵涌 卞修武 |
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| 作者单位： | 重庆医科大学基础医学院病理生理学教研室,重庆,400016;第三军医大学西南医院病理学研究所,重庆,400038 |
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| 摘 要： | 目的 研究诺帝(Nordy)对HPV16型亚基因(E6、E7)永生化人宫颈上皮细胞(H8细胞)的诱导分化作用.方法 MTT法检测诺帝对H8细胞增殖的抑制作用,流式细胞术(FCM)分析细胞周期,免疫细胞化学S-P法检测增殖细胞核抗原Ki67的表达,TRAP-ELISA法检测端粒酶活性变化.结果 诺帝能够明显抑制H8细胞增殖;可阻滞细胞周期于G0/G1期,S期细胞减少;细胞核内Ki67蛋白的表达水平明显下降;端粒酶活性明显降低.结论 诺帝对H8细胞有诱导分化作用,这种作用可能是通过抑制增殖,阻滞细胞周期,降低端粒酶活性来实现的.
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| 关 键 词： | 诱导分化 宫颈 永生化 HPV |
| 文章编号： | 1000-5404（2007）01-0043-04 |
| 修稿时间： | 2006-01-19 |
Nordy on induced differentiation of HPV16 subgenes-immortalized human endocervical cells |
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| ZHAO Jing,ZHAO Yong,BIAN Xiu-wu.Nordy on induced differentiation of HPV16 subgenes-immortalized human endocervical cells[J].Acta Academiae Medicinae Militaris Tertiae,2007,29(1):43-46. |
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| Authors: | ZHAO Jing ZHAO Yong BIAN Xiu-wu |
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| Affiliation: | 1.Department of Pathophysiology, Chongqing Medical University, Chongqing 400016 ;2.Department of Pathology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China |
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| Abstract: | Objective To investigate the effects of Nordy on induced differentiation of HPV16 subgenes-immortalized human endocervical cells(H8 cells).Methods After treated with Nordy,the proliferation of H8 was tested with MTT assay.The effect of Nordy on cell cycle of H8 was analyzed by flow cytometer(FCM).The expression of Ki67 of H8 was detected by immunohistochemistry.The activity of telomerase was tested by TRAP-ELISA.Results Nordy could inhibit proliferation of H8 cells significantly.H8 cells were arrested in G_(0)/G_(1) phase after Nordy treatment.The Ki67 expression and telomerase activity reduced significantly after Nordy treatment.Conclusion Nordy could induce the differentiation of H8 cell lines by inhibiting their proliferation,blocking cell cycle and decreasing telomerase activity. |
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| Keywords: | HPV |
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