| 桂枝茯苓丸诱导卵巢癌HO8910细胞凋亡的研究 |
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| 引用本文： | 王英,高洪泉. 桂枝茯苓丸诱导卵巢癌HO8910细胞凋亡的研究[J]. 牡丹江医学院学报, 2003, 24(6): 1-4 |
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| 作者姓名： | 王英 高洪泉 |
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| 作者单位： | 牡丹江医学院,157011;牡丹江医学院,157011 |
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| 基金项目： | 黑龙江省教育厅科学技术研究项目(10 5 3 3 0 0 5 ) |
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| 摘 要： | 目的：探讨桂枝茯苓丸对卵巢癌细胞株HO8910的抑制增殖效应及凋亡诱导作用。方法：用桂枝茯苓丸处理HO8910细胞，采用MTT法检测药物对细胞的抑制作用，倒置显微镜观察细胞形态学改变，以及应用流式细胞仪观察细胞凋亡过程中细胞周期的变化。结果：在桂枝茯苓丸作用下，HO8910细胞呈凋亡改变，DNA琼脂糖凝胶电泳呈典型的凋亡特征。细胞凋亡的同时，细胞周期发生特定的改变。结论：桂枝茯苓丸能抑制卵巢癌细胞增殖，能诱导卵巢癌细胞凋亡。
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| 关 键 词： | 桂枝茯苓丸(GFW) 细胞凋亡 卵巢癌细胞株 |
| 修稿时间： | 2003-11-09 |
STUDIES OF GFW ON INDUCING APOPTOSIS IN OVARIAN CANCER CELL LINES HO8910 |
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| Wang Ying Mudanjiang Medical College. STUDIES OF GFW ON INDUCING APOPTOSIS IN OVARIAN CANCER CELL LINES HO8910[J]. Journal of Mudanjiang Medical College, 2003, 24(6): 1-4 |
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| Authors: | Wang Ying Mudanjiang Medical College |
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| Affiliation: | Wang Ying Mudanjiang Medical College 157011 |
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| Abstract: | Objective: The current study was designed to investigate whether GFW can induces apoptosis in Ovarian Cancer cell.Methods:Cell proliferation was assessed by MTT assays.Morphological changes of apoptosis were observed with invert microscope . Cell cycle were examined by DNA agarose gel eletronphores and PI fluorescence flow cytometry(FCM),respectively.Results:Under the influence of GFW, HO8910 cell exhibited changes of apoptosis both in morphology and in the characteristics of electrophoresis.Meanwhile the cell cycle also showed special change.Conclusion: GFW can inhibit cell proliferation, GFW can induces apoptosis in Ovarian Cancer cell. |
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| Keywords: | GFW apoptosis ovarian cancer cell line |
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